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Date Published: 27/10/2023
Abstract Views: 155
Views PDF: 131
DOI: https://doi.org/10.56535/jmpm.v48i8.446
Issue
Vol. 48 No. 8 (2023): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 8 - 2023
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Articles
How to Cite
Nguyễn, T. P., Phùng, T. H., Nguyễn, H. P., Nguyễn, N. T., Hoàng, T. K., Lê, V., Đỗ, A. T., Lê, V. Đ., & Đỗ, K. Đ. (2023). A PROTOCOL FOR THE PURIFICATION AND EXPANSION OF PERIPHERAL BLOOD NATURAL KILLER CELLS DERIVED FROM PROSTATE CANCER PATIENTS. Journal of Military Pharmaco-medicine, 48(8), 16-25. https://doi.org/10.56535/jmpm.v48i8.446

A PROTOCOL FOR THE PURIFICATION AND EXPANSION OF PERIPHERAL BLOOD NATURAL KILLER CELLS DERIVED FROM PROSTATE CANCER PATIENTS

Nguyễn Trọng Phúc, Thế Hải Phùng, Hoàng Phương Nguyễn, Ngọc Tuấn Nguyễn, Trung Kiên Hoàng, Việt Lê, Anh Tuấn Đỗ, Văn Đông Lê, Khắc Đại Đỗ1,
1 dokhacdai@vmmu.edu.vn

Main Article Content

Abstract

Objectives: To establish a protocol for the purification and expansion of Natural Killer cells (NK cells) derived from prostate cancer patients in vitro. Methods: We collected peripheral blood samples from 3 patients diagnosed with prostate cancer for peripheral blood mononuclear cells (PBMCs) separation and generated high-purity NK cells by using Microbeads (Miltenyi/Germany) from the PBMCs (day 0 - D0). The NK cells were cultured in a KBM-specific medium (Kohjin-Bio/Japan) for expansion and harvested on day 14 (D14). Results: The purification rate of  NK cells was 93.4% on day D0 and 94.9% on day D14. The average number of harvested NK cells at D14 was 385.3 x 106 cells (which increased by 192.7 times than that of day D0). Conclusion: We have successfully established and implemented successfully the process of purifying and culturing NK cells in prostate cancer patients.

Article Details

Keywords

Prostate cancer, NK cells, Purification process, Proliferation process

References

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