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Date Published: 28/09/2023
Abstract Views: 150
Views PDF: 151
DOI: https://doi.org/10.56535/jmpm.v48i7.412
Issue
Vol. 48 No. 7 (2023): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 7 - 2023
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Articles
How to Cite
Nguyễn, T. H. T., Nguyễn, M. Q., Hoàng, T. N. D., Hoàng, T. H., Đỗ, N. B., Nguyễn, K. L., & Lê, Q. T. (2023). APPLICATION OF RECOMBINANT TES-30 ANTIGEN TO DETECT IgG ANTIBODY IN SERA OF TOXOCARIASIS PATIENTS BY ELISA TECHNIQUE. Journal of Military Pharmaco-medicine, 48(7), 84-92. https://doi.org/10.56535/jmpm.v48i7.412

APPLICATION OF RECOMBINANT TES-30 ANTIGEN TO DETECT IgG ANTIBODY IN SERA OF TOXOCARIASIS PATIENTS BY ELISA TECHNIQUE

Thị Hà Trinh Nguyễn, Minh Quyền Nguyễn, Thị Ngọc Diệp Hoàng, Thị Hòa Hoàng, Như Bình Đỗ, Khắc Lực Nguyễn, Quốc Tuấn Lê1,
1 Học viện Quân y

Main Article Content

Abstract

Objectives: To use recombinant TES protein to improve the quality of the ELISA kit detecting anti-TES IgG in the infected sera to lessen the disadvantages of raw TES-based ELISA kit as low sensitivity and specificity. Methods: Descriptive experimental study in the laboratory using recombinant TES-30 (rTES-30) protein expressed in E. coli BL21 (DE3) to develop an ELISA kit to detect IgG antibody in the sera of infected patients. Results: A recombinant TES-based ELISA kit has been produced. The concentration of recombinant TES-30 protein was 1 µg/mL and the cut-off value was 0.4. The sandwich ELISA using rTES-30 possessed a sensitive level reaching 93.3% and a specificity of 100%. Conclusion: A recombinant TES-based ELISA kit has been successfully developed for human anti-TES IgG detection. This recombinant protein held great potential for developing diagnostic tests for Toxocariasis in Vietnam.

Article Details

Keywords

Toxocara spp. excretory/secretory antigen, Recombinant protein TES-30, Expression

References

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