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Date Published: 22/08/2023
Abstract Views: 261
Views PDF: 216
DOI: https://doi.org/10.56535/jmpm.v48i6.388
Issue
Vol. 48 No. 6 (2023): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 6 - 2023
Section
Articles
How to Cite
Hoàng, X. C., Đỗ, N. B., Vũ, M. T., Bùi, T. L., Nguyễn Thị , T. T., Trương, C. Đ., Bùi, M. T., Bùi, T. N., Lâm, T. N., & Võ, T. B. T. (2023). CLONING AND EXPRESSION OF GENE ENCODING RECOMBINANT NS1-DENV1-4 SEROTYPE ANTIGEN IN ESCHERICHIA COLI. Journal of Military Pharmaco-medicine, 48(6), 16-23. https://doi.org/10.56535/jmpm.v48i6.388

CLONING AND EXPRESSION OF GENE ENCODING RECOMBINANT NS1-DENV1-4 SEROTYPE ANTIGEN IN ESCHERICHIA COLI

Xuân Cường Hoàng, Như Bình Đỗ, Minh Thương Vũ, Thùy Linh Bùi, Thanh Thảo Nguyễn Thị , Công Định Trương, Minh Trường Bùi, Thế Ngọc Bùi, Thảo Nguyên Lâm, Võ Thị Bích Thủy

Main Article Content

Abstract

Objectives: To study the cloning, expression, and purification of the general NS1 antigen with epitope-detectable antibodies against four Dengue virus (DENV) serotypes. Methods: The gene coding for NS1 antigen generally four serotypes was inserted into pET22b+ vector then transformed into E.coli BL21. Recombinant products were examined by PCR, restriction enzyme, sequencing, and SDS-PAGE methods. Results: The gene encoding the recombinant NS1 antigen with a size of 417 bp, 139 amino acids, and 100% homology with the polypeptide sequence of the NS1 antigen was published on GenBank. SDS-PAGE electrophoresis results showed that the recombinant protein 6xHis-rNS1 has a molecular weight of approximately 18 kDa. Conclusion: Successful expressed the recombinant NS1 antigen in E.coli. It was the initial result of research aimed at creating monoclonal antibodies to detect four serotype DENVs in Vietnam.

Article Details

Keywords

Dengue virus, E.coli, NS1, Recombinant antigen

References

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