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DETERMINATION OF THE CHEMICAL COMPOSITION AND BIOACTIVITY OF THAN PHUC ((HOMALOMENA VIETNAMENSIS J. BOGNER ET V.D.NGUYEN), FAMILY (ARACEAE))
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Abstract
Objectives: To determine the chemical composition and biological activity of Than Phuc (Homalomena vietnamensis J. Bogner et V.D.Nguyen), family (Araceae)). Subjects and methods: The rhizomes of Than Phuc are collected in Dong Giang-Quang Nam. Preliminary determination of the composition by chemical reactions; Extraction of essential oils by steam distillation method, composition determination, and quantification by gas chromatography-mass spectrometry (GC/MS). The antimicrobial activity of the test is assessed through the turbidity of the culture medium. The antioxidant activity is investigated by DPPH free radical scavenging method. Results: Than phuc rhizomes have much essential oil, triterpenoids, tannins, and reducing agent. The main components of Than phuc essential oil are β-Linalool (71,19%), α-cadinol (8,10%), terpinen-4-ol (4.80%), tau-muurolol (4.89%). IC50 value for E.coli strain (2.51 ± 0.37 mg/mL), B.subtilis (2.87 ± 0.09 mg/mL), L.fermentum (3.33 ± 0.05 mg/mL), S.aureus (3.73 ± 0.04 mg/mL), C.albican (3.42 ± 0.05 mg/mL), P.aeruginosa (6.30 ± 0.17 mg/mL), S.enterica (3.56 ± 0.26 mg/mL). MIC at a dilution concentration of 5.65 mg/mL was able to inhibit 99% E.coli, 97% C.albican, 94% B.subtili, L.fermentum, and 88% S.aureus and at a dilution concentration of 22.58 mg/mL it has the ability to inhibit 100% S.enterica. The highest MBC values are for C.albican 97% and Gram (-) E.coli 99% at a dilution of 5.65 mg/mL. At a dilution concentration of 90.30 mg/mL, Than phuc essential oil inhibits 100% S.enterica and 99% S.aureus. EC50 value 58.05 ± 3.1 mg/mL. Conclusion: The main chemical composition of Than phuc is essential oil, in which monoterpenes without oxygen account for 4.43%, monoterpenes with oxygen account for 76.88%, sesquiterpenes make up 17.17%; essential oil has inhibitory effects (S.aureus, B.subtilis, L.fermentum, P.aeruginosa), bactericidal (E.coli and C.albican fungi) and has very weak antioxidant effects.
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Keywords
Than phuc, Homalomena vietnamensis, Biological activity, Antimicrobial, Antioxidant
References
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