NGHIÊN CỨU ĐẶC ĐIỂM VI KHUẨN ĐỜM BẰNG KỸ THUẬT PCR ĐA MỒI Ở BỆNH NHÂN MẮC BỆNH PHỔI TẮC NGHẼN MẠN TÍNH ĐỢT CẤP ĐIỀU TRỊ TẠI BỆNH VIỆN QUÂN Y 103

Abstract

Objectives: To describe the clinical characteristics and sputum bacterial profiles using multiplex PCR in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) treated at Military Hospital 103. Methods: A prospective, cross-sectional descriptive study was conducted on 35 inpatients with AECOPD at the Respiratory Center, Military Hospital 103, from July 2024 to April 2025. Results: The mean age of the study group was 73.1 ± 7.0 years, with a male predominance of 94.3%. Fever was present in 62.9% of patients, and 37.1% had pneumonia. Increased dyspnea was the most common symptom (97.1%). Multiplex PCR detected a total of 37 bacteria, with S. pneumoniae accounting for the highest proportion (32.4%). Multiplex PCR detected bacteria in 74.3% of patients, consistent with culture results (100%). There was no difference in clinical characteristics between the groups of patients with positive and negative multiplex PCR (p > 0.05). Conclusion: AECOPD patients were predominantly elderly males with multiple comorbidities and a variety of symptoms. S. pneumoniae was the most frequently detected bacterium. The bacterial detection proportion by the multiplex PCR technique was high and consistent with semi-quantitative bacterial culture results.