NGHIÊN CỨU GIÁ TRỊ CỦA METHYL HOÁ GENE SEPT9 HUYẾT TƯƠNG TRONG CHẨN ĐOÁN UNG THƯ ĐẠI TRỰC TRÀNG

Abstract

Objectives: To study the value of methylation of the SEPT9 gene (mSEPT9) in peripheral blood for the diagnosis of colorectal cancer (CRC). Methods: A cross-sectional descriptive study involving 244 participants, including 92 healthy individuals undergoing health checks at Military Hospital 103 and 152 patients with CRC monitored at Military Hospital 103 and K Hospital from January 2021 to December 2024. The detection of mSEPT9 in peripheral blood was determined using the Nested-PCR technique with an extended sequence probe (ExBP). Results: The early detection value of CRC using mSEPT9 differentiates CRC patients from healthy individuals with a sensitivity of 76.31%, specificity of 89,13%, positive predictive value of 92,06%, and negative predictive value of 69,49%. ROC curve analysis recorded an AUC of 0,827 for the diagnostic value of CRC using mSEPT9, with p < 0.0001. Individuals with positive mSEPT9 are at a 26,42 times higher risk of CRC compared to those with negative mSEPT9 (p < 0.0001). In the CRC group, patients with positive mSEPT9 have a 3,719 times higher risk of progressing to late-stage disease compared to those with negative mSEPT9 (p < 0.001). Conclusion: mSEPT9 demonstrates high sensitivity and specificity as a diagnostic tool for CRC and its convenience and non-invasive nature play a role in improving the quality of early screening for CRC. Keywords: Colorectal cancer, SEPT9 gene methylation.