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XÂY DỰNG VÀ THẨM ĐỊNH PHƯƠNG PHÁP ĐỊNH LƯỢNG QUERCETIN TRONG CÂY TRAI HOA TRẦN (MURDANNIA NUDIFLORA (L) BRENAN) BẰNG SẮC KÝ LỎNG HIỆU NĂNG CAO
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Abstract
Objectives: Development and validattion a high-performance liquid chromatography method for the quantification of Quercetin in Murdannia nudiflora (L.). Methods: Surveying, validating, and selecting chromatographic condition method according to ICH guidelines. Results: The chromatographic conditions were determined including column Optimapak C18 (250 mm x4.6mm; 5µm), mobile phase: MeOH – acid formic 0.2% (30:70) in isocratic with flow rate of 0.7 ml/min, injection volume of 20 μL, and detector PDA-UV at 370 nm. The method was validated with high specificity, linearity, precision and accuracy, and low limit of quantitation. The result showed the content of Quercetin in Murdannia nudiflora (L.) ranged from 6.18 to 11.70 mg/g depending on extraction methods. Conclusion: The determination of HPLC method for quantification of Quercetin in Murdannia nudiflora (L.). This is the background for further studies
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Keywords
Murdannia nudiflora, Quercetin, HPLC
References
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