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Date Published: 28/02/2024
Abstract Views: 141
Views PDF: 89
DOI: https://doi.org/10.56535/jmpm.v49i2.720
Issue
Vol. 49 No. 2 (2024): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 2 - 2024
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Articles
How to Cite
Nguyễn, M. N., Nguyễn , T. T. D., Đinh , T. T. H., Nguyễn , Đ. D., Bùi , L. A., Sklan, E., & Hoàng, V. T. (2024). HVQY75-ESTABLISHING THE STANDARD CURVES TO QUANTIFY LONG NON-CODING RNA IFI6lnc2 AND EPB41L4A-AS1 CONCENTRATION USING REAL-TIME PCR METHOD. Journal of Military Pharmaco-medicine, 49(2), 245-255. https://doi.org/10.56535/jmpm.v49i2.720

HVQY75-ESTABLISHING THE STANDARD CURVES TO QUANTIFY LONG NON-CODING RNA IFI6lnc2 AND EPB41L4A-AS1 CONCENTRATION USING REAL-TIME PCR METHOD

Minh Nam Nguyễn1, Nguyễn Thị Thùy Dịu, Đinh Thị Thu Hằng, Nguyễn Đăng Dũng, Bùi Lan Anh, Ella Sklan, Hoàng Văn Tổng
1 Khoa Truyền Nhiễm - Bệnh viện Quân y 103

Main Article Content

Abstract

Abstract


Objectives: The study aims to establish the standard curves for quantifying the concentrations of LncRNA IFI6lnc2 and EPB41L4A-AS1. Methods: The absolute quantitative real-time PCR method was employed to construct the standard curves based on standard samples derived from recombinant plasmids containing the coding genes for these lncRNAs. Results: The coding genes for lncRNAs IFI6lnc2 and EPB41L4A-AS1 were cloned successfully in pJET1.2 by conventional recombinant methods. The recombinant plasmids exhibited high concentrations, with pJET-IFI6lnc2 at a concentration of 1.8 x 1010 copy/μl and pJET-EPB41L4A-AS1 at a concentration of 2.4 x 1010 copy/μl. Standard curve equation of lncRNA IFI6lnc2: Y=-3.408X + 35.989 (R2=0.998, E =96.5%) and lncRNA EPB41L4A-AS1: Y= -3.4706X + 36.225 (R2=0.999, E =94.0%). Conclusion: The results of the standard curves demonstrated a linear correlation and high amplification efficiency.

Article Details

Keywords

EPB41L4A-AS1, IFI6lnc2, standard curve, Long non-coding RNA (LncRNA)

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