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ỨNG DỤNG KHÁNG NGUYÊN NS1 TÁI TỔ HỢP GỘP BỐN TÝP VIRUS DENGUE ĐỂ PHÁT HIỆN KHÁNG THỂ IgM/IgG TRONG MÁU BỆNH NHÂN MẮC SỐT XUẤT HUYẾT DENGUE
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Abstract
The dengue virus, transmitted by Aedes spp mosquitoes and encompassing four serotypes (DEN-1 to DEN-4), causes dengue fever. The biomarker NS1 plays a pivotal role in diagnosing dengue fever through specific immune tests. Methods: The methodology includes employing an Indirect ELISA with the recombinant antigen rAgNS1-DENV1-4, capable of identifying IgM/IgG antibodies for all four dengue serotypes in patient blood samples. This method compares the sensitivity and specificity with realtime PCR. Results: The Indirect ELISA immune tests utilizing the rAgNS1-DENV1-4 antigen demonstrate a sensitivity of 95.9% and specificity of 99.33%, with a cut-off threshold of 0.353. The positive predictive value is 95.21%, and the negative predictive value is 99.43%. Conclusion: The recombinant antigen rAgNS1-DENV1-4 successfully attains a dependable threshold for detecting IgM/IgG antibodies in Dengue hemorrhagic fever.
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Keywords
NS1, Indriect ELISA, Sensitivity, Specificity
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