NGHIÊN CỨU BÀO CHẾ HỆ NANO LIPID RẮN CHỨA CELECOXIB VÀ ĐÁNH GIÁ KHẢ NĂNG ỨC CHẾ MỘT SỐ DÒNG TẾ BÀO UNG THƯ VÚ IN VITRO

Abstract

Objective: Prepare and evaluate the anti-cancer effect on cells of a solid lipid nanoparticle containing celecoxib (CXB). Method: Solid lipid nanoparticle containing celecoxib was prepared by homogenization method combined with ultrasonication. The obtained formulations were evaluated for properties such as nanoparticle size, zeta potential, differential scanning calorimetry (DSC), etc., and evaluated for anti-cancer effects on some cancer cell lines. Results: The final solid lipid nanoparticle contained 10 mg celecoxib, 50 mg stearic acid, 50 mg Compritol ATO 888 in 20 mL water containing 1% soluplus. The selected formulation has a particle size of 75.1 ± 0.97 nm, polydisperse index (PDI) <0.3, and encapsulation efficiency of 83.5% ± 0.03. The anticancer effect effects were obtained on some breast cancer lines: MDA-MB-231, MCF-7, and SK-BR-3. The formulation was stable after 3 months of storage at 4^oC. Conclusion: The study developed and evaluated properties and parameters of a solid lipid nanoparticle containing celecoxib. In addition, the in vitro anticancer effects on some breast cancer cell lines were also evaluated.