Article Sidebar
Views PDF: 89
The results of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of newly pulmonary tuberculosis
Main Article Content
Abstract
Objective: To evaluate the results of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of newly pulmonary tuberculosis. Subjects and methods: A prospective, descriptive, cross-sectional study was conducted on 40 pulmonary tuberculosis patients and 20 community-acquired pneumonia patients, treated as inpatient at the Respiratory Center, Military Hospital 103, from September 2021 to November 2022. Evaluating the sensitivity and specificity of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of pulmonary tuberculosis, comparing with the results of the GeneXpert/Mtb-Rif test. Results: Results of 16S rRNA-real-time ExBP-RT-PCR test were positive in 37 patients (92.5%). The sensitivity, specificity, positive predictive value, and negative predictive value of the test are 92.5%, 100%, 100%, and 86.95%, respectively. The positive proportion of the 16S rRNA-real-time ExBP-RT-PCR test is higher than GeneXpert/Mtb-Rif but not statistically significant (p>0.05). Conclusions: 16S rRNA-real-time ExBP-RT-PCR test has high sensitivity, specificity, positive predictive value, and negative predictive value in diagnosis of newly pulmonary tuberculosis.
Keywords: Pulmonary tuberculosis; 16S rRNA; PCR.
Article Details
Keywords
Pulmonary tuberculosis, 16S rRNA, PCR
References
2. Cattamanchi A, Dowdy DW, Davis JL, et al. Sensitivity of direct versus concentrated sputum smear microscopy in HIV-infected patients suspected of having pulmonary tuberculosis. BMC Infect Dis. 2009; 9(1):53.
3. Pang Y, Lu J, Su B, et al. Misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by GeneXpert MTB/RIF assay. Infection. 2017; 45(5):677-681.
4. Emerson JB, Adams RI, Román CMB et al. Schrödinger’s microbes: Tools for distinguishing the living from the dead in microbial ecosystems. Microbiome 5. 2017; 86.
5. Bộ Y tế. Hướng dẫn chẩn đoán, điều trị và dự phòng bệnh lao. 2020.
6. Mandell LA, Wunderink RG, Anzueto A, et al. infectious diseases society of America/American thoracic society consensus guidelines on the management of community-acquired pneumonia in adults. Clinical infectious diseases: An official publication of the infectious diseases society of America. 2007; 44 Suppl 2(Suppl 2):27-72.
7. Phạm Ngọc Hảo. Nghiên cứu giá trị của lâm sàng, X-quang phổi chuẩn và PCR trong chẩn đoán lao phổi AFB tính. Luận văn cao học, Đại học Y Hà Nội. 2013.
8. Kang W, Du J, Yang S, et al. The prevalence and risks of major comorbidities among inpatients with pulmonary tuberculosis in China from a gender and age perspective: A large-scale multicenter observational study. Eur J Clin Microbiol Infect Dis. 2021; 40:787-800.
9. Zhiyi L. The Value of GeneXpert MTB/RIF for detection in tuberculosis: A bibliometrics-based analysis and review. J Anal Methods Chem. 2022 Oct ;15:2915018.
10. Horita N, Yamamoto M, Sato T, et al. Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: Meta-analysis of 26999 specimens from 17 Studies. Sci Rep 5. 2016; 18113.
11. Choi Y, Hong SR, Jeon BY, et al. Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex. Int J Tuberc Lung Dis. 2015; 19:1102-1108.
12. Huh HJ, Koh WJ, Song DJ, et al. Evaluation of the Cobas TaqMan MTB test for the detection of Mycobacterium tuberculosis complex according to acid-fast-bacillus smear grades in respiratory specimens. Journal of clinical microbiology. 2015; 53(2):696-698.