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The results of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of newly pulmonary tuberculosis
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Abstract
Objective: To evaluate the results of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of newly pulmonary tuberculosis. Subjects and methods: A prospective, descriptive, cross-sectional study was conducted on 40 pulmonary tuberculosis patients and 20 community-acquired pneumonia patients, treated as inpatient at the Respiratory Center, Military Hospital 103, from September 2021 to November 2022. Evaluating the sensitivity and specificity of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of pulmonary tuberculosis, comparing with the results of the GeneXpert/Mtb-Rif test. Results: Results of 16S rRNA-real-time ExBP-RT-PCR test were positive in 37 patients (92.5%). The sensitivity, specificity, positive predictive value, and negative predictive value of the test are 92.5%, 100%, 100%, and 86.95%, respectively. The positive proportion of the 16S rRNA-real-time ExBP-RT-PCR test is higher than GeneXpert/Mtb-Rif but not statistically significant (p>0.05). Conclusions: 16S rRNA-real-time ExBP-RT-PCR test has high sensitivity, specificity, positive predictive value, and negative predictive value in diagnosis of newly pulmonary tuberculosis.
Keywords: Pulmonary tuberculosis; 16S rRNA; PCR.
Article Details
Keywords
Pulmonary tuberculosis, 16S rRNA, PCR
References
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