NGHIÊN CỨU BÀO CHẾ NANO RUTIN BẰNG PHƯƠNG PHÁP KẾT TỦA TRONG DUNG MÔI

Abstract

Objectives: To prepare the rutin nanoparticles using the anti-solvent precipitation method and evaluate some physicochemical properties of the obtained particles. Methods: The anti-solvent precipitation method was applied to prepare nano rutin and the solvent was removed using spray drying method. The prepared nano rutin was evaluated for some properties such as particle size, polydispersity index, and zeta potential. Results: The formulation of rutin nanoparticles was built, including rutin (concentration of 10 mg/mL in methanol), HPMC E15 (concentration of 0,8% in water); surfactant is sodium lauryl sulfate (NaLS, concentration 0,2% in water); methanol/water ratio (1/10, v/v). Some in-processing parameters were selected, including the fluid injection speed (120 drops/minute), the magnetic stirring speed (1500 rpm), stirring time (5 minutes); then continued sonication in 5 minutes. After spray drying, the nanoparticle was in yellow powder form with fine size and high porosity. When redispersion in water, the particle size of rutin nano was less than 250nm with the polydispersity index less than 0.5, and the zeta potential was -37,2 ± 2,3mV. Conclusion: Rutin nanoparticles were successfully prepared by the anti-solvent precipitation method and evaluated some physicochemical properties of the obtained particles.