NGHIÊN CỨU TỔNG HỢP MOLNUPIRAVIR TỪ CYTIDIN

Abstract

Objectives: To synthesize molnupiravir in laboratory conditions from cytidine with a simple method and no need for column chromatography for purification. Methods: Four different reactions were used to synthesize molnupiravir from cytidine as the starting material. Acetonidation of cytidine to produce C1; Acylation of C1 to produce C2; Hydroxylamination of C2 to produce C3; and hydrolysis of C3 to produce molnupiravir. The fundamental techniques for product refinement included distributing extraction, crystallization, precipitation, filtering, and washing. The structures of the compounds were confirmed by spectral analysis methods such as IR, MS, and NMR. Results: Yield 96.0% of C1 from cytidine, 90.6% of C2 from C1, 59.0% of C3 from C2, and 44.6% of the final molnupiravir from C3 were obtained based on the yield of the synthesis stages. The overall efficiency of the process was 22.9%, and it used non-column chromatography to isolate products that were pure according to thin-layer chromatography and melting point. Spectral data provided a complete characterization of the structure of compounds. Conclusion: Molnupiravir was successfully synthesized in the lab using an easy, safe, and potentially efficient method.