NGHIÊN CỨU XÂY DỰNG MÔ HÌNH XƠ GAN VÀ ĐÁNH GIÁ TÁC DỤNG CỦA VIÊN NANG NHẤT GAN LINH TRÊN ĐỘNG VẬT THỰC NGHIỆM

Abstract

Objectives: To establish a liver fibrosis model in BALB/c mice using CCl₄ with optimal duration and dosage, and evaluate the therapeutic effects of NGL capsules on liver fibrosis. Methods: Liver fibrosis was induced in BALB/c mice by subcutaneous injection of CCl4 at a dose of 160mg/kg diluted in olive oil at a ratio of 1:18, corresponding to a dose of 2 mL/kg x 2 times per week for 4, 8, and 12 weeks. After liver fibrosis induction, the mice were orally administered NGL capsules at doses ranging from 150 - 450 mg/kg/day. The effectiveness of liver fibrosis treatment was assessed based on changes in body weight, survival rate, hematological and biochemical parameters, as well as histopathological analysis. Results: The CCl₄-induced liver fibrosis model in BALB/c mice demonstrated fibrotic stages of 1/4, 2/4, 2/4, and 3/4, corresponding to the duration of CCl₄ administration for 4, 8, and 12 weeks. Mice treated with NGL capsules at clinical equivalent dose, double and triple the clinical dose, all showed the ability to improve liver injury at the biochemical and cellular levels. Conclusion: NGL has demonstrated its therapeutic effects on liver fibrosis in the established model of liver fibrosis in BALB/c mice induced by subcutaneous injection of CCl4 at a dose of 2 mL/kg x 2 times per week for 8 weeks.