NGHIÊN CỨU ĐỘC TÍNH CẤP VÀ BÁN TRƯỜNG DIỄN CỦA VIÊN NANG MỀM HUP A TRÊN ĐỘNG VẬT THỰC NGHIỆM

Abstract

Objectives: To evaluate the acute toxicity on Hup A soft capsules in mice and sub-chronic toxicity in rabbits. Methods: The acute toxicity was assessed in mice using the Litchfield - Wilcoxon method; the semi-chronic toxicity was assessed in rabbits according to the guidelines of the OECD and the Ministry of Health of Vietnam. The products used in this study were Hup A soft capsules, Enterprise standard. Results: After giving Hup A capsules (40.00 - 160.00 g/kg) to mice, we determined LD50 = 92.88 g/kg (79.59 - 108.39 g/kg). High dose batches of mice showed tremors, increased sweating, and then they died. With two dosage of 0.12 g/kg/24h and 0.36 g/kg/24h, rabbits were administered continuously during 42 days. The results showed that no impact was detected on some hematological parameters: Red blood cell count, white blood cell count, and hemoglobin content; indicators of liver and kidney function: AST, ALT enzyme activities, Cholesterol, Albumin, Total Bilirubin and Creatinine content, histopathology of liver and kidney cells were normal in experimental rabbits. Conclusion: Hup A capsules had LD50 = 92,88 g/kg (79,59 - 108,39 g/kg) in white mice and did not affect hematopoietic, liver, or kidney function of experimental rabbits after 42 days of study.