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Date Published: 28/02/2024
Abstract Views: 123
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DOI: https://doi.org/10.56535/jmpm.v49i2.490
Issue
Vol. 49 No. 2 (2024): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 2 - 2024
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Articles
How to Cite
Đỗ, N. B., Hoàng, X. C., Trần, T. H. T., Võ, T. B. T., Trịnh, T. S., & Pham, H. T. (2024). APPLICATION OF MONOCLONAL ANTIBODY mAbNS1-DENV1-4 TO DETECT NS1 ANTIGEN OF FOUR DENGUE TYPES OF VIRUS BY ELISA METHODS. Journal of Military Pharmaco-medicine, 49(2), 174-185. https://doi.org/10.56535/jmpm.v49i2.490

APPLICATION OF MONOCLONAL ANTIBODY mAbNS1-DENV1-4 TO DETECT NS1 ANTIGEN OF FOUR DENGUE TYPES OF VIRUS BY ELISA METHODS

Đỗ Như Bình, Xuân Cường Hoàng, Thị Hồng Thịnh Trần, Võ Thị Bích Thủy , Thế Sơn Trịnh, Pham Hung Tien1,
1 Trường Đại học Y tế công cộng

Main Article Content

Abstract

Dengue hemorrhagic fever is caused by the Dengue Virus (DENV), transmitted by the mosquito Aedes spp. Serology tests using the ELISA technique with monoclonal antibodies made from recombinant NS1 antigens of four types DENV 1-4 is a valuable tool in disease detection. Methods: Monoclonal antibody mAbNS1-DENV specific to NS1 of four dengue virus types was used to develop and optimize the indirect ELISA test, allowing accurate detection of DENV 1-4 in patient sera. Comparison of sensitivity and specificity with real-time PCR. Results: ELISA technique using monoclonal antibody mAbNS1-DENV1-4 has 100% sensitivity, 97.75% specificity, 98.37% positive prediction and 100% negative prediction. Furthermore, this assay specifically detects NS1 antibodies in both NS1 DENV 4 antigens in the group of suspected samples (9/63). Conclusion: The monoclonal antibody mABNS1-DENV1-4 is valuable for rapid diagnosis of DENV types with high sensitivity and specificity.

Article Details

Keywords

Dengue hemorrhagic fever, Monoclonal antibodies, Sensitivity, Specificity

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