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PREPARATION AND BIOACTIVITY EVALUATION OF MANGOSTEEN PEEL (GARCINIA MANGOSTANA L.) DRY EXTRACT IN VIETNAM
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Abstract
Objectives: To prepare of polyphenol-rich extracts from mangosteen peels (Garcinia mangostana L.), and evaluate the antioxidant activity, α-glucosidase inhibitory effects, and anti-Propionibacterium acnes (P.acnes) activity of the extracts. Methods: The mangosteen peels were cleaned, dried, ground, and extracted by ultrasound-assisted method with the investigated parameters: Solvent, temperature, extraction time, and powder-to-solvent ratios. The antioxidant activity was evaluated by the DPPH method. The α-glucosidase inhibitory activity was tested using the p-NPG substrate method. The antibacterial activity was assessed by resazurin broth microdilution assay. Results: Polyphenol-rich mangosteen peel extracts were successfully prepared (195.05 ± 4.01mg GAE/g) by ultrasound-assisted heating with 80% ethanol, 70°C, 150 minutes, and the powder-to-solvent ratio of 1/15 g/mL. The exract had antioxidant and α-glucosidase inhibitory activity with IC50 of 35 µg/mL and 269.07 µg/mL, respectively. The anti-P. acnes activity of the extract with MIC and MBC were 7.81 µg/mL and 31.2 µg/mL. Conclusion: The process for preparing extracts from mangosteen peels with high polyphenol content was developed. The antioxidant, α-glucosidase inhibitory effects, and anti-P. acnes activity of the extract was evaluated.
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Keywords
Mangosteen peel, Garcinia mangostana L., Polyphenol, Antioxidant, α-glucosidase, Propionibacterium acnes
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