NGHIÊN CỨU TRIỂN KHAI MÔ HÌNH GÂY SỎI TIẾT NIỆU THỂ THẤP NHIỆT HẠ CHÚ TRÊN CHUỘT CỐNG TRẮNG

Abstract

Objectives: To develop a calcium oxalate urolithiasis model with damp-heat downward flow syndrome in Wistar rats. Methods: An randomized controlled experimental study was conducted on 30 Wistar rats, randomly divided into 3 groups (n = 10): Group 1 (the control group): Received distilled water; group 2 (the urolithiasis group): Received an oral solution of ethylene glycol (EG) 0.75% (v/v) and ammonium chloride (AC) 2% (w/v) in drinking water; and group 3 (the urolithiasis combined with damp-heat downward flow syndrome group): Received the same lithogenic treatment combined with a humid-heat environment, a high-lipid diet, and restricted activity. The modeling period lasted 28 days. Body weight, urinary biochemical parameters, hematological indices, renal function indices, inflammatory cytokines, gross renal findings, and renal histopathology H&E staining were evaluated. Results: Rats in the model groups showed lower weight gain compared with the control group. Urinary oxalate, calcium, phosphate, serum creatinine, blood urea nitrogen (BUN), CRP, IL-6, and TNF-α increased, whereas urinary pH decreased significantly. Calcium oxalate crystals were detected in 80% of rats in group 2 and 100% in group 3. Histopathological examination revealed calcium oxalate crystal deposition in renal tubules. Conclusion: The calcium oxalate urolithiasis model combined with damp-heat downward flow syndrome was successfully established.