NGHIÊN CỨU THÀNH PHẦN HÓA HỌC VÀ KHẢO SÁT HOẠT TÍNH SINH HỌC CỦA TINH DẦU CHIẾT XUẤT TỪ LOÀI PRAXELIS CLEMATIDEA R. M. KING & H. ROBINSON

Abstract

Objectives: To extract the essential oil from Praxelis clematidea and analyze its chemical composition. To investigate the antibacterial and cytotoxic activities of the extracted essential oil. Subjects and methods: The essential oil was extracted from Praxelis clematidea by steam distillation. The chemical composition of the extracted essential oil was analyzed by gas chromatography coupled mass spectrometry (GC-MS). The antibacterial activity of the essential oil was investigated by the agar diffusion method, plus the minimum inhibitory concentration determination on pathogenic bacteria. The cytotoxic activity of the essential oil was evaluated in vitro in human cell lines by total protein staining with sulforhodamine B. Results: Essential oil extracted from Praxelis clematidea has a chemical composition of 20 main compounds, of which 7 have not been identified yet. The essential oil exhibited antibacterial activity against Acinetobacter baumannii, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecium, Pseudomonas aeruginosa with MIC value ranging from 2 - 16 mg/mL. The extracted essential oil exhibited cytotoxic activity against lung cancer cell line NCI H460 with the IC₅₀ value of 73.01 ± 2.37 and the selective index value of 1.23. Conclusion: The essential oil extracted from Praxelis clematidea R. M. King & H. Robinson consists of 20 chemical compounds. The essential oil has cytotoxic activity and especially antibacterial activity against antibiotic-resistant pathogenic bacteria.