ĐÁNH GIÁ TÍNH AN TOÀN CỦA BÀI THUỐC TIÊU PHONG THANG TRÊN ĐỘNG VẬT THỰC NGHIỆM

Abstract

Objectives:To determine the acute safety threshold and evaluate the sub-chronic toxicity of Tieu phong thang (TPT) remedy by assessing its effects on general condition, hematological, biochemical parameters, and histopathology of the liver and kidneys in experimental animals. Methods: Acute toxicity was investigated in Swiss mice to determine the LD₅₀. For subchronic toxicity, 30 Wistar rats were randomly divided into 3 groups (n = 10 per group): A control group (distilled water), treatment group 1 (TPT at a dose of 17.05 g/kg/day), and treatment group 2 (TPT at a dose of 51.15 g/kg/day). Parameters were evaluated at D0, D14, and D28. Histopathology of the liver and kidneys was assessed on day 28. Results: The LD₅₀ of TPT in Swiss mice could not be determined at the maximum oral dose (375 g/kg). In the sub-chronic study, the remedy did not alter clinical manifestations or body weight gain (p > 0.05). Hematological parameters (RBC, HGB, HCT, WBC, PLT) and biochemical parameters (AST, ALT, creatinine, albumin, total cholesterol) showed no statistically significant differences between the treatment groups and the control group (p> 0.05). No gross or microscopic lesions were recorded in the liver or kidneys. Conclusion: TPT oral solution exhibited no acute toxicity and achieved sub-chronic safety at the investigated doses.