KHẢO SÁT HOẠT TÍNH CHỐNG OXY HÓA IN VITRO VÀ ĐỘC TÍNH BÁN TRƯỜNG DIỄN CỦA CAO CHIẾT MẠCH MÔN (OPHIOPOGON JAPONICUS) TRÊN ĐỘNG VẬT THỰC NGHIỆM

Abstract

Objectives: To assess the antioxidant properties and subchronic toxicity of Ophiopogon japonicus extract (CCMM). Methods: The in vitro antioxidant activity was determined using the DPPH radical scavenging assay, while subchronic toxicity was examined in Wistar rats administered oral doses of 1.2 and 3.6 g/kg/day for 28 days.Results: The CCMM demonstrated the ability to inhibit DPPH free radicals in a concentration-dependent manner, with an IC50 of 47.95 µg/mL. During the toxicity assessment, no mortality or clinical abnormalities were recorded; the body weight of the subjects increased consistently across the groups. Hematological and biochemical parameters, including Hb, Hct, and albumin, exhibited minor variations from baseline values that were not statistically significant as compared to the control group at the corresponding time point. Histopathological examinations of the liver and kidneys reveal no lesions associated with the test samples. Conclusion: CCMM exhibits notable antioxidant activity and is well-tolerated over 28 days, thereby providing a scientific foundation for its long-term therapeutic applications.