INITIAL INVESTIGATION ON SFRP5 EXPRESSION IN DIET-INDUCED OBESE MICE
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Abstract
Objectives: To evaluate the impact of the diet on SFRP5 gene expression, as well as to examine its temporal and spatial expression patterns in various adipose tissue sites in mice. Methods: 50 mice were divided into four groups and fed with either a standard diet (STD) or a high-fat diet (HFD) for 60 and 100 days using RT-qPCR after extracting total mRNA from mouse adipose tissue at three sites (interscapular, inguinal, and epididymal adipose tissue). Results: The results showed that HFD consumption led to an increase in SFRP5 expression in all three adipose tissue sites. Furthermore, 100-day-HFD exposure also up-regulated SFRP5 expression in inguinal white adipose tissue compared to the 60-day period. We also found that epididymal white adipose tissue exhibited the most prominent SFRP5 expression among the tissues studied, suggesting that epididymal adipose tissue is a potential site for examining SFRP5 gene expression in mice. Conclusion: The research findings provide further evidence that SFRP5 is a valuable marker for early prediction of adipose tissue hypertrophy and can be used to monitor obesity treatment outcomes.
Keywords
Obesity, Experimental mice, High-fat diet, SFRP5 gene, Gene expression
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References
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