ĐÁNH GIÁ SỰ THAY ĐỔI HÀM LƯỢNG COLLAGEN TẠI MÔ VẾT THƯƠNG MẠN TÍNH THỰC NGHIỆM ĐƯỢC ĐIỀU TRỊ BẰNG HUYẾT TƯƠNG GIÀU TIỂU CẦU TỪ MÁU CUỐNG RỐN NGƯỜI

Abstract

Objectives: To evaluate collagen content changes in experimental chronic wound tissue treated with human umbilical cord blood–derived platelet-rich plasma (hUCB-PRP). Methods: A controlled experimental and longitudinal study was conducted on 30 Sprague Dawley rats (300 - 450g) with induced chronic wounds using adriamycin, divided into 3 groups: A negative control group (NaCl 0.9%), an investigation group (hUCB-PRP), and a positive control group (Heberprot-P). Local treatment was administered at a dose of 0.2mL of hUCB-PRP per wound on days D5 and D10. Collagen proliferation was assessed through hydroxyproline (Hyp) quantification at time points D0, D5, and D21, combined with histological evaluation using Masson's tricolor staining at D21. Results: The average Hyp content of normal rat skin was 43.34 ± 3.58 mg/g of tissue. After adriamycin including the chronic wound model, Hyp levels markedly decreased and reached the lowest value at D5 (6.14 ± 0.72 mg/g of tissue). At D21, the hUCB-PRP-treated group showed significantly higher hydroxyproline levels than the negative control group (16.80 ± 2.95 vs. 9.61 ± 0.52 mg/g tissue; p < 0.001). Masson’s trichrome staining demonstrated a marked increase in collagen deposition, with collagen fibers being thicker and more evenly arranged in the treated group. Conclusion: hUCB-PRP stimulates collagen proliferation and improves collagen structure in an adriamycin-induced chronic wound experimental model.