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Date Published: 10/12/2025
Abstract Views: 15
Views PDF: 3
DOI: 10.56535/jmpm.v50si4.1517
Issue
Vol. 50 No. si4 (2025): JOURNAL OF MILITARY PHARMACO-MEDICINE - SPECIAL ISSUE 12/2025
Section
Special issue celebrating the 75th anniversary of Military Hospital 103
How to Cite
Do, N. L., Nguyen, T. T., Trinh, T. S., Nguyen, T. T. A., Le, T. H., & Dao, N. H. (2025). EFFICACY OF THE MODIFIED VITRIFICATION TECHNIQUE WITH AND WITHOUT CRYOPROTECTIVE AGENTS IN LOW SPERM COUNT SAMPLES. Journal of Military Pharmaco-medicine, 50(si4), 453-460. https://doi.org/10.56535/jmpm.v50si4.1517

EFFICACY OF THE MODIFIED VITRIFICATION TECHNIQUE WITH AND WITHOUT CRYOPROTECTIVE AGENTS IN LOW SPERM COUNT SAMPLES

Ngoc Lan Do1, , Thanh Tung Nguyen1, The Son Trinh1, Thi Thuc Anh Nguyen1, Thanh Huyen Le1, Nguyen Hung Dao2
1 Viện Mô phôi Lâm sàng Quân đội, Học viện Quân y
2 Bệnh viện Quân y 103, Học viện Quân y

Main Article Content

Abstract

Objectives: To compare the effectiveness of the modified sperm vitrification technique with and without the use of cryoprotective agents (CPA). Methods: An experimental, laboratory study was conducted on 30 oligospermia and 12 azoospermia aspirated from the epididymis treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University. Results: In both oligospermia and azoospermia samples (low sperm count samples), the viable and motile cryosurvival factors (CSF) in the CPA group were statistically significantly higher than those in the no-CPA group (p < 0,05). Conclusion: Vitrification of low sperm count samples in PCR tubes using CPA resulted in better sperm survival and mobility after thawing compared to the no-CPA group.

Keywords

Sperm vitrification, Oligospermia, Azoospermia, CPA, CSF

Article Details

References

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