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Date Published: 28/10/2025
Abstract Views: 25
Views PDF: 14
DOI: 10.56535/jmpm.v50i8.1422
Issue
Vol. 50 No. 8 (2025): JOURNAL OF MILITARY PHARMACO-MEDICINE No.8 - 2025
Section
Articles
How to Cite
Nguyen, M. T., Nguyễn, T. T. T., Dang, T. M., Nguyen, V. P., Ta, Q. T., Nguyen, V. B., Nguyen, T. H., Nguyen, V. H., Nguyen, T. M. N., & Nguyen, V. C. (2025). DEVELOPMENT OF A SANDWICH LATERAL FLOW IMMUNOASSAY TO DETECT OCHRATOXIN A IN CORN AND RICE. Journal of Military Pharmaco-medicine, 50(8), 43-52. https://doi.org/10.56535/jmpm.v50i8.1422

DEVELOPMENT OF A SANDWICH LATERAL FLOW IMMUNOASSAY TO DETECT OCHRATOXIN A IN CORN AND RICE

Manh Tuan Nguyen1, , Thị Thu Trang Nguyễn2, Tuan Minh Dang2, Van Phong Nguyen2, Quang Thanh Ta3, Van Bang Nguyen4, Trong Ha Nguyen4, Van Hong Nguyen1, Thi Minh Ngoc Nguyen5, Van Chuyen Nguyen2
1 Viện Sốt rét - Ký sinh trùng - Côn trùng Trung ương
2 Học viện Quân y
3 Bệnh viện Bắc Thăng Long
4 Bệnh viện Quân y 103, Học viện Quân y
5 Đại học Y Dược Hải Phòng

Main Article Content

Abstract

Objectives: To fabricate a highly sensitive sandwich-based lateral flow immunoassay (LFIA) for the detection of OTA in corn and rice. Methods: To produce a sandwich LFIA, OTA toxin will be attached to BSA and converted into OTA-BSA. OTA-BSA is captured by both anti-BSA antibody on the conjugate pad and anti-OTA on the test line to form a sandwich structure. Results: The limits of LFIA for the detection were 4.0 µg/L for Ochratoxin A in Phosphate Buffered Saline (PBS) buffer, and the repeatability was 5/5 for 5 tested concentrations of OTA. The stability of LFIA was 3 months when it was stored at 4°C and 25°C. Conclusion: A sandwich-based LFIA for rapid detection of OTA in corn and rice was successfully developed.

Keywords

Lateral flow immunoassay, Sandwich, Ochratoxin A

Article Details

References

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