Article Sidebar

PDF
Date Published: 28/09/2025
Abstract Views: 14
Views PDF: 8
DOI: https://doi.org/10.56535/jmpm.v50i7.1337
Issue
Vol. 50 No. 7 (2025): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 7 - 2025
Section
Articles
How to Cite
Bùi, T. P. H., Đoàn , V. K., Phùng , M. P., Đoàn , N. H., Nguyễn , M. Đ., & Lương , X. H. (2025). TOTAL SYNTHESIS AND BIOLOGICAL ACTIVITY EVALUATION OF THE PEPTIDE Cn-AMP3 DERIVED FROM COCOS NUCIFERA WATER. Journal of Military Pharmaco-medicine, 50(7), 62-71. https://doi.org/10.56535/jmpm.v50i7.1337

TOTAL SYNTHESIS AND BIOLOGICAL ACTIVITY EVALUATION OF THE PEPTIDE Cn-AMP3 DERIVED FROM COCOS NUCIFERA WATER

Thị Phương Hải Bùi1, Văn Kể Đoàn 1, Minh Phượng Phùng 1, Ngân Hoa Đoàn 1, Minh Đức Nguyễn 1, Xuân Huy Lương 1,
1 Đại học Phenikaa

Main Article Content

Abstract

Objectives: To perform the total synthesis of the bioactive peptide Cn-AMP3 at laboratory scale and evaluate its antibacterial activity and hemolytic potential. Methods: Cn-AMP3 was synthesized using solid-phase peptide synthesis (SPPS). The biological activity of the peptide was assessed via broth microdilution and hemolysis assays. Results: Cn-AMP3 was successfully synthesized with a yield of 43.8% and a purity exceeding 98%. The peptide exhibited weak antibacterial activity against Klebsiella pneumoniae and Staphylococcus aureus. However, its efficacy was significantly enhanced when combined with the antibiotic kanamycin. Regarding safety, Cn-AMP3 induced less than 5% hemolysis at the highest tested concentration of 256 µM. Conclusion: The peptide Cn-AMP3 was successfully synthesized and preliminarily evaluated for its antibacterial and hemolytic activities, which suggested its potential for use in combination therapies with conventional antibiotics.

Article Details

Keywords

Antimicrobial peptides, Solid-phase peptide synthesis, Cn-AMP3

References

1. RM Zellweger, J Carrique-Mas, D Limmathurotsakul, et al. A current perspective on antimicrobial resistance in Southeast Asia. Journal of Antimicrobial Chemotherapy. 2017; 72(11):2963-2972, https://doi.org/10.1093/ jac/dkx260.
2. M Hayes and S Bleakley. 21-Peptides from plants and their applications. Peptide Applications in Biomedicine, Biotechnology and Bioengineering. 2018:603-622. https://doi.org/10.1016/ B978-0-08-100736-5.00025-9.
3. MJ Santana, AL de Oliveira, LHK Queiroz Júnior, et al. Structural insights into Cn-AMP1, a short disulfide-free multifunctional peptide from green coconut water. FEBS Letters. 2015; 589(5):639-644, https://doi.org/10.1016/ j.febslet.2015.01.029.
4. HD Ngan, BL Huy, CL Uyen, et al. Antimicrobial peptides in green coconut water: from nutritional benefits to multifunctional applications for human health. European Food Research and Technology. 2025. https://doi.org/10.1007/ s00217-025-04754-6.
5. A Narayanankutty, JT Job, A Moothakoottil Kuttithodi, et al. Proximate composition, antioxidant, anti-inflammatory and anti-diabetic properties of the haustorium from Coconut (Cocos nucifera L.) and Palmyra palm (Borassus flabellifer L.). Journal of King Saud University - Science. 2023; 35(1):102404, https://doi.org/ 10.1016/j.jksus.2022.102404.
6. European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. 2003; 9:ix-xv. https://doi.org/10.1046/ j.1469-0691.2003.00790.x
7. Luong HX, Kim DH, Lee BJ, et al. Antimicrobial and hemolytic activity of stapled heptapeptide dimers. Bulletin of the Korean Chemical Society. 2016; 37(8):1199-1203. https://doi.org/10.1002/ bkcs.10839.
8. Bùi Thị Phương Hải, Đoàn Ngân Hoa, Hoàng Văn Hải, Trương Thanh Tùng, Lương Xuân Huy. Nghiên cứu tổng hợp toàn phần hoạt chất pentapeptide-3. Tạp chí Y Dược học Quân sự. 2024; 49(6):17-27. https://doi.org/ 10.56535/jmpm.v49i6.488.