NGHIÊN CỨU ẢNH HƯỞNG CỦA THÔNG SỐ QUY TRÌNH ĐẾN ĐIỀU CHẾ DỊCH CHIẾT TỪ BÀI THUỐC KIỆN VỊ BỔ TRUNG

Abstract

Objectives: To investigate the influence of process parameters on the preparation of liquid extract from the traditional herbal formula Kien vi bo trung. Methods: The formula was divided into two groups for extraction using ethanol and purified water, respectively. Key extraction variables, including solvent type, solvent-to-material ratio, temperature, and extraction time, were evaluated. The extraction yield of Atractylenolide (ATL) III and total water-soluble components were selected as evaluation criteria to determine optimal extraction conditions and then develop an extraction process. Results: Ethanol extraction phase, increasing ethanol concentration and the extraction temperature significantly enhanced the extraction yield of ATL III. The solvent-to-material ratio (10:1 to 40:1, v/w) showed minimal effect. The highest ATL III yield was achieved at 2 hours of extraction, while prolonged extraction to 3 hours resulted in a decreased yield. Aqueous extraction phase: Optimal conditions were identified as a solvent-to-material ratio of 10:1 (v/w), with extraction performed at boiling temperature for 2 hours. Conclusion:The study successfully identified appropriate process parameters for extracting the Kien vi bo trung formula. These findings provide a scientific foundation for the development of a standardized extraction process, supporting the modernization of traditional formulations into pharmaceutical dosage forms.