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EFFECT OF BIOPSY TECHNOLOGY ON BLASTOCYST MORPHOLOGY IN PREIMPLANTATION GENETIC TESTING
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Abstract
Abstract
Objectives: Evaluate the changes in morphology, and structure of blastocysts before biopsy, 3 hours after the biopsy, and 4 hours after thawing at the Military Institute of Clinical Embryology and Histology, Military Medical University.
Subjects and methods: The descriptive and prospective study on 623 blastocysts of 186 infertile patients undergoing In Vitro Fertilization treatment combines Preimplantation Genetic Testing. Based on maternal age, 623 blastocysts were divided into 3 groups: <30, 30-35, and >35 years of age. All blastocysts were frozen by the vitrification method 3 hours after the biopsy.
Results: 3 hours after the biopsy, 605/623 re-expanded (97,1%), the blastocyst's medium diameter was significantly smaller than before the biopsy in general and in each group in particular (p<0,05), however, there was no statistically significant difference in the zona-pellucida’s thickness of the blastocyst between two-time points (p=0,06). 105 euploid blastocysts were thawed. 4 hours after warming, 100% of the blastocyst survived and re-expanded, the blastocyst’s average diameter increased and the thickness of zona pellucida decreased significantly compared to before the biopsy in the whole 3 groups (p<0,001).
Results: 3 hours after the biopsy, 97,1% of blastocysts re-expanded. The blastocyst's medium diameter was significantly smaller than before the biopsy in general and in each group in particular. 4 hours after warming, 100% of the blastocyst survived and re-expanded, the blastocyst’s average diameter increased and the thickness of zona pellucida decreased significantly compared to before the biopsy in the whole 3 groups.
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Keywords
Keywords: Blastocyst biopsy, Diameter of blastocysts, Morphology of biopsied blastocyst…
References
2. Cimadomo D, Capalbo A, Levi-Setti PE, et al. Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation. Hum Reprod. 2018; 33(11):1992-2001.
3. Zhao H, Tao W, Li M, et al. Comparison of two protocols of blastocyst biopsy submitted to preimplantation genetic testing for aneuploidies: A randomized controlled trial. Arch Gynecol Obstet. 2019; 299(5):1487-1493.
4. Đoàn Thị Hằng. Nghiên cứu biến đổi hình thái cấu trúc phôi người ngày 3, ngày 5 trước đông lạnh và sau rã đông bằng kỹ thuật thuỷ tinh hoá. Luận án Tiến sĩ Y học, Học viện Quân y. 2013.
5. Dương Đình Hiếu. Nghiên cứu hình thái và đánh giá liên tục phôi 3 và 5 ngày tuổi của bệnh nhân thụ tinh trong ống nghiệm. Luận án Tiến sĩ Y học, Học viện Quân y. 2016.
6. Kirkegaard K, Hindkjaer JJ, Ingerslev HJ. Human embryonic development after blastomere removal: A time-lapse analysis. Hum Reprod. 2012; 27(1):97-105.
7. Coello A, Meseguer M, Galán A, et al. Analysis of the morphological dynamics of blastocysts after vitrification/ warming: Defining new predictive variables of implantation. Fertil Steril. 2017; 108(4):659-666.e654.
8. Scott RT Jr, Upham KM, Forman EJ, et al. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: A randomized and paired clinical trial. Fertil Steril. 2013; 100(3):624-630.
9. Capalbo A, Ubaldi FM, Cimadomo D, et al. Consistent and reproducible outcomes of blastocyst biopsy and aneuploidy screening across different biopsy practitioners: A multicentre study involving 2586 embryo biopsies. Hum Reprod. 2016; 31(1):199-208.