XÁC ĐỊNH LOÀI VÀ PHÂN TÍCH ĐA HÌNH DI TRUYỀN GENE COX1 CỦA SÁN LÁ GAN NHỎ THU THẬP TỪ NGƯỜI TẠI MỘT XÃ THUỘC TỈNH YÊN BÁI NĂM 2020

Abstract

Objectives: This study was conducted to identify species and analyze the genetic diversity of C. sinensis in Yen Bai province, Vietnam, based on the complete 1560 bp sequence of the mitochondrial cox1 gene. Methods: Twenty small liver fluke individuals were collected from humans in Xuan Long commune, Yen Binh district, Yen Bai province. Ten of them were sequenced to obtain the complete 1560 bp sequence of the mitochondrial cox1 gene. Subsequently, the isolated cox1 sequences were compared with reference sequences (FJ381664.2) from the GenBank to analyze genetic diversity. Results: Twenty-three polymorphic sites in the cox1 gene sequences were identified at positions 66, 114, 243, 300, 321, 381, 384, 414, 465, 471, 933, 954, 981, 1041, 1071, 1137, 1181, 1244, 1323, 1380, 1428, 1449, 1494, and 1503. The nucleotide substitution at position 1244 resulted in a Threonine → Methionine amino acid substitution. The cox1 gene was AT-rich, with an estimated AT/GC ratio ranging from 1.44 to 1.46. Conclusion: The mitochondrial cox1 gene sequences are important molecular markers used in genetic diversity analysis and phylogenetic tree reconstruction of the small liver fluke.