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Ngày xuất bản: 28/04/2025
Số lượt xem tóm tắt: 11
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DOI: https://doi.org/10.56535/jmpm.v50i4.931
Số xuất bản
Tập 50 Số 4 (2025): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 4 - 2025
Chuyên mục
Bài báo
Trích dẫn bài báo
Phung, T. H., Vu, T. T., Doan, H. T., Nguyen, H. P., Trinh, N. L., Hoang, T. K., Nguyen, N. T., Vu, A. H., Nguyen, D. D., & Do, K. D. (2025). OPTIMIZATION OF THE FLOW CYTOMETRIC METHOD FOR ANALYZING NK CELL CYTOTOXIC ACTIVITY IN BREAST CANCER. Tạp chí Y Dược học Quân sự, 50(4), 32-40. https://doi.org/10.56535/jmpm.v50i4.931

OPTIMIZATION OF THE FLOW CYTOMETRIC METHOD FOR ANALYZING NK CELL CYTOTOXIC ACTIVITY IN BREAST CANCER

Phung The Hai1, Vu Thi Trang2, Doan Ha Trang1, Nguyen Hoang Phuong1, Trinh Ngoc Linh1, Hoang Trung Kien1, Nguyen Ngoc Tuan1, Vu Anh Hai2, Nguyen Dang Dung1, Do Khac Dai1,
1 Department of Immunology, Vietnam Military Medical University
2 Department of Oncology, Military Hospital 103, Vietnam Military Medical University

Nội dung chính của bài viết

Tóm tắt

Objectives: To establish a protocol for analyzing NK cell cytotoxic activity (NKAc) in breast cancer patients using the flow cytometric technique. Methods: We first optimized the protocol based on the previously published studies, focusing on choosing fluorescent dye concentration, incubation time length, and Effector:Target cell ratio (E:T ratio). Subsequently, we preliminarily applied the established protocol to characterize NKAc in some breast cancer patients and healthy controls. Lastly, we compared the NKAc versus NK cell secretory activity (NKAs) data obtained from the study. Results: The fluorescent dye concentration could be used according to the manufacturer's suggestion (CFSE: 2.5 - 10µM; Zombie NIR: 1:1000 - 1:100 dilution). The co-culture period of effector and target cells and the E:T ratio could be 4 hours and 5:1, respectively. NKAc was reproducible for 1 month. 2/7 breast cancer patients had NKAs < 200 pg/mL and NKAc < 10%. Conclusion: It is feasible to examine NKAc in breast cancer patients via the flow cytometric method established in this study. Further studies are needed to validate the diagnostic and prognostic value of NK cell function tests.

Chi tiết bài viết

Từ khóa

NK cell cytotoxic activity, NK cell secretory activity, Flow cytometry, Breast cancer

Tài liệu tham khảo

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