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Ngày xuất bản: 26/04/2024
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Số lượt xem PDF: 49
DOI: https://doi.org/10.56535/jmpm.v49i4.588
Số xuất bản
Tập 49 Số 4 (2024): TẠP CHÍ Y DƯỢC HỌC QUÂN SỰ SỐ 4 - 2024
Chuyên mục
Bài báo
Trích dẫn bài báo
Nguyen, T. T., Tran, V. T., Do, N. L., Nguyen, T. T. A., Ho, S. H., & Trinh, T. S. (2024). INITIAL EVALUATION OF THE EFFICIENCY OF CRYOPROTECTANT-FREE VITRIFICATION IN PCR TUBES ON A LOW NUMBER OF HUMAN SPERMATOZOA. Tạp chí Y Dược học Quân sự, 49(4), 5-12. https://doi.org/10.56535/jmpm.v49i4.588

INITIAL EVALUATION OF THE EFFICIENCY OF CRYOPROTECTANT-FREE VITRIFICATION IN PCR TUBES ON A LOW NUMBER OF HUMAN SPERMATOZOA

Nguyen Thanh Tung1, Tran Van Tuan1, Do Ngoc Lan1, Nguyen Thi Thuc Anh1, Ho Sy Hung2, Trinh The Son1,
1 Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University
2 Hanoi Medical University

Nội dung chính của bài viết

Tóm tắt

Objectives: To inititally evaluate the efficiency of cryoprotectant-free vitrification in PCR tubes on a low number of human spermatozoa. Methods: A laboratory experimental study was conducted on 30 severe oligospermia treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University. The semen samples were vitrified and thawed at the following times, then sperm concentration, morphology, viability, and motility were evaluated after thawing. Results: The viable CSF, motile CSF when vitrifing without using CPAs were 50.45 ± 4.63; 43.35 ± 4.81 (1-week storage), 45.24 ± 4.33; 37.24 ± 4.39 (4-week storage) and 40.69 ± 3.96; 33.96 ± 4.14 (8-week storage), respectively. Conclusion: Vitrification of human sperm can be achieved without cryoprotectants and could be recommended for routine assisted reproductive technology.

Chi tiết bài viết

Từ khóa

Sperm vitrification, Oligospermia, Free-cryoprotectants

Tài liệu tham khảo

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