Truong Thi Thu Hien1,, Do Thanh Tuan2, Nguyen Thi Thu Hien3, Truong Duc Minh4, Vu Khanh LInh4
1 Vietnam Military Medical University
2 Thai Binh University of Medicine and Pharmacy
3 Hanoi University of Mining and Geology
4 Phenikaa University

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Tóm tắt

Objectives: To isolate and determine the chemical structure of some compounds from the roots of Schisandra Cauliflora and their inhibitory activity on nitric oxide production. Methods: The roots of Schisandra cauliflora, which were collected in Vinh Phuc in April 2022, were processed before being extracted and isolated to obtain compounds. Compounds structures were elucidated based on extensive spectroscopic analyses, including 1D- and 2D-nuclear magnetic resonance spectroscopy (NMR), mass spectrometry (HR-ESI-MS), and CD spectra. The obtained compounds were evaluated for inhibitory activity on nitric oxide production on LPS stimulated RAW 264.7 cells. Results and conclusion: From the methanol extract of the roots of Schisandra cauliflora, three dibenzocyclooctadiene lignans were isolated, including ananonin J, ananolignan F, and ananolignan C. Their structures were elucidated. All the compounds inhibited nitric oxide productions on LPS stimulated RAW 264.7 cells with IC50 values of 45.24 ± 1.46, 41.32 ± 1.45, and 48.71 ± 1.34µM, respectively, compared to that of the positive control of dexamethasone (IC50 14.20 ± 0.54µM). This is the first time these lignan compounds have been isolated from the roots of Schisandra cauliflora.

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Tài liệu tham khảo

1. Võ Văn Chi. Từ điển Cây thuốc Việt Nam. Nhà Xuất bản Y học 2012. Tập 1.
2. Phạm Hoàng Hộ. Cây cỏ Việt nam. Nhà Xuất bản trẻ 1999. Tập 2.
3. Szopa A, Barnas M, Ekiert H, Phytochemical studies and biological activity of three Chinese Schisandra species (Schisandra sphenanthera, Schisandra henryi and Schisandra rubriflora): Current findings and future applications. Phytochemistry Reviews. 2019; 18: 109-128.
4. Yang S, Yuan C. Schisandra chinensis: A comprehensive review on its phytochemicals and biological activities. Arabian Journal of Chemistry. 2021; 14(9): 103310.
5. Opletal L, Sovova H, Bártlova M. Dibenzo[a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination. Journal of Chromatography B. 2004; 812(1-2): 357-371.
6. Mai N T, Doan V V, Lan H T T, Anh B T M, Hoang N H, Tai B H, Nhiem N X, Yen P H, Park S J, Seo Y, Namkung W, Kim S H, Kiem P V. Chemical constituents from Schisandra sphenanthera and their cytotoxic activity. Natural Products Research. 2021; 35(20): 3360-3369.
7. Cuong N T, Hai D V, Hung N Q, Dat M H. Schisandra cauliflora (Schisandraceae), a new species from Vietnam. Blumea - Biodiversity, Evolution and Biogeography of Plants. 2019; 64(2): 183-185.
8. Yang J H, Zhang H Y, Du X, Wang W, Xiao W L, Wen J, Pu J X, Tang X C, Sun H D. New dibenzocyclooctadiene lignans from the Kadsura ananosma. Tetrahedron. 2011; 67(25): 4498-4504.
9. Yang J H, Zhang H Y, Wen J, Du X, Chen J H, Zhang H B, Xiao W L, Pu J X, T ang X C, Sun H D. Dibenzocyclooctadiene lignans with antineurodegenerative potential from Kadsura ananosma. Journal of Natural Products. 2011; 74(5): 1028-1035.
10. Su Yeon Oh, Young Hoon Kim, Deok Sung Bae, Byung Hun Um, Cheol Ho Pan, Chul Young Kim, Hee Ju Lee,Jae Kwon Lee. Anti-Inflammatory Effects of Gomisin N, Gomisin J, and Schisandrin C Isolated from the Fruit of Schisandra chinensis. Bioscience, Biotechnology, and Biochemistry. 2010; 74(2): 285-291.