Journal of Military Pharmaco-medicine

DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF PARACETAMOL AND METHOCARBAMOL IN TABLETS

2024-12-24T08:00:33+00:00

Objective: To develop and validate a procedure for the simultaneous quantification of Paracetamol and Methocarbamol in tablets by high performance liquid chromatography (HPLC) in order to contribute to drug quality control. Subjects and methods: Quantitative Paracetamol and Methocarbamol by HPLC method with LunaR C18 (4 × 250 mm, 5µm), column temperature 26°C, flow rate 1.0 ml/min, detector UV at 274 nm, injection volume 10 µl, mobile phase consisting of acetonitril and 0.2 % formic acid in water with a gradient program. Results: The validation results for suitability, specificity, linearity, precision, and accuracy showed that this method was conformed to the ICH requirements. Conclusion: The quantitative method meets the requirements and can be used for the simultaneous quantification of Paracetamol and Methocarbamol in tablets. * Keywords: Paracetamol, Methocarbamol, HPLC, tablets.

APPLICATION OF GIS TECHNOLOGY IN ESTABLISHING DATABASE FOR GEOSPATIAL EPIDEMIOLOGY AND ANALYSIS OF RELATED FACTORS TO INFECTIOUS DISEASE IN THE NORTHERN MIDLANDS AND MOUNTAINOUS REGIONS (2014 - 2023)

2025-03-10T10:15:37+00:00

Objectives: To set up a geospatial epidemiological database of infectious diseases and factors related to infectious diseases in the northern midland and mountainous provinces in the period from 2014 to 2023. Methods: Data were processed using Excel and MapInfo software, a mapping method for creating thematic maps. Results: 2,080 survey forms of all kinds were edited and processed; 353,635 database data records of geospatial epidemiological map attributes of infectious diseases and factors related to infectious diseases in the northern midland and mountainous provinces were digitized and updated in the period from 2014 to 2023 using MapInfo software. Exploiting and applying MapInfo software to build thematic maps of a number of infectious diseases including: respiratory transmission, gastrointestinal transmission, bloodborne pathogen transmission under the national expanded immunisation programme. Conclusion: Building a geospatial epidemiological database of infectious diseases in the northern midland and mountainous regions has provided an overall picture including information on morbidity and mortality of infectious diseases along with the relevance of certain natural features; socio-economic; animals, plants, microorganisms and infectious diseases in strategic areas of the Fatherland.

ĐÁNH GIÁ ĐỘC TÍNH CẤP VÀ ẢNH HƯỞNG CỦA CHẾ PHẨM BỔ PHẾ NGỌC THÁI ĐẾN THỂ TRẠNG CHUNG VÀ CÁC CHỈ SỐ HUYẾT HỌC TRÊN ĐỘNG VẬT THỰC NGHIỆM

2025-03-25T07:00:53+00:00

Mục tiêu: Đánh giá độc tính cấp của chế phẩm Bổ phế Ngọc Thái trên chuột nhắt trắng Swiss và xác định ảnh hưởng của chế phẩm đến thể trạng chung, chỉ số huyết học của chuột cống trắng Wistar. Phương pháp nghiên cứu: Nghiên cứu xác định độc tính cấp theo phương pháp Litchfield-Wilcoxon bằng cách cho chuột nhắt trắng Swiss uống chế phẩm theo liều tăng dần, theo dõi tỷ lệ chuột chết và dấu hiệu bất thường trong vòng 7 ngày. Ảnh hưởng của chế phẩm đến thể trạng và chỉ số huyết học được đánh giá trên chuột cống trắng theo hướng dẫn của Bộ Y tế, dùng với liều 7 mL/kg/ngày và 21 mL/kg/ngày, theo dõi trong 28 ngày. Các chỉ số huyết học gồm hồng cầu, bạch cầu, tiểu cầu, huyết sắc tố, hematocrit và thể tích trung bình hồng cầu được phân tích tại các thời điểm trước khi uống thuốc (D0), sau 14 ngày (D14) và sau 28 ngày (D28). Kết quả: Chuột nhắt trắng khi uống chế phẩm Bổ phế Ngọc Thái ở mức liều tối đa 150 mL/kg thể trọng trong 24 giờ không có chuột nào chết. Chuột cống trắng được uống chế phẩm với liều 7 mL/kg/ngày và 21 mL/kg/ngày không làm thay đổi đến thể trạng chung cũng như các chỉ số huyết học giữa các lô chuột nghiên cứu. Kết luận: Chế phẩm Bổ phế Ngọc Thái không gây độc tính cấp và không ảnh hưởng đáng kể đến thể trạng chung, các chỉ số huyết học khi đánh giá trên động vật thực nghiệm.

Study on optimizing production of CAR-T cell mass

2025-03-11T07:25:10+00:00

Objective: Optimizing the co-culture process of CAR-T cells and aAPC 1D2 to obtain the highest quantity and quality of CAR-T cells. Subjects and methods:Separating PBMC from fresh blood samples. Then PBMC was analyzed by BD Facslyric flow cytometer. Transposition into PBMC using Amaxa Nucleofector 2b with T-020 program. Co-culture CAR-T and aAPC at a ratio of 2X viable CAR-T cells. CAR expression was analyzed by real-time PCR. Results:On 7th and 14th day, the number of CAR-T cells proliferated many times higher than that on 3rd day. The proliferative capacity of the two CAR-T cell generations was similar on 14th day and there was a difference of that from day 21. After 28th day, the number of iCasp9-IL15 CAR-T cells (78.62%) was higher than that of CD19RCD137 CAR-T cells (66.23%) with p>0.05. The results on day 28 showed that approximately 97% of CAR-T cells of both cell generations expressed CAR on the surface after proliferation. Conclusion:CAR-T cells proliferated effectively from 28th day on co-culture with artificial antigen-presenting cells and IL-2 with aAPC supplementation of 4 times (1st, 7th, 14th, 21st day). the obtained cells expressed CAR molecules of 97% on the surface.

ASSESSMENT OF ACUTE TOXICITY AND EFFECTS ON HEMATOLOGICAL PARAMETERS OF DINH TAM NGOC THAI PREPARATION IN AN EXPERIMENTAL RESEARCH MODEL

2025-04-01T00:35:11+00:00

Objective : To evaluate the acute toxicity in Swiss albino mice and the effects on hematological parameters in Wistar rats of the Dinh Tam Ngoc Thai preparation. Methods : Acute toxicity was assessed using the Litchfield–Wilcoxon method. The effects of the preparation on hematological parameters were evaluated according to WHO guidelines. Dosages were calculated in milliliters of the preparation. Results : Swiss albino mice were orally administered Dinh Tam Ngoc Thai at doses ranging from 25 ml/kg (equivalent to 186.90 g of medicinal herbs/kg body weight) to 150 ml/kg (equivalent to 101.40 g of medicinal herbs/kg body weight). After monitoring for 72 hours and up to 7 days post-administration, no mortality was observed. In Wistar rats that were given Dinh Tam Ngoc Thai for 28 consecutive days at doses of 7 ml/kg/day (equivalent to 4.732 g of medicinal herbs/kg/day) and 21 ml/kg/day (equivalent to 14.196 g of medicinal herbs/kg/day), no effects on hematological parameters were recorded. Conclusion : Dinh Tam Ngoc Thai is safe in acute toxicity evaluation in Swiss albino mice and does not affect hematological parameters when administered continuously for 28 days in Wistar rats

Transposition of Sleeping beauty plasmid and minicircle into T cells using Nucleofector technology

2025-03-30T12:49:02+00:00

Objective: Study on the transposition of Sleeping Beauty plasmid and minicircle in the transposition of chimeric antigen receptors into T cells using Nucleofector technology. Subjects and methods: Fresh blood samples were processed and separated peripheral blood mononuclear cells (PBMC), then this cells were counted and analyzed by flow cytometry, the transpositions of plasmid and minicircle were conduct to create CAR-T. Finally, the transposition efficiency was evaluated by flow cytometry analysis. Results: The transposition efficiency of the second generation CAR-T CD19RCD137/pSB plasmid construct (25.88%) was higher than that of the fourth-generation iCasp9-IL15/pSB CAR-T (14.72%), and the minicircle CAR-T CD19RCD137/pSB (35.12%) was higher than that of the iCasp9-IL15/pSB CAR-T (23.48%) with p<0.05. The viable cell rate of the second-generation CD19RCD137/pSB CAR-T (48.9%) was higher than that of the fourth-generation iCasp9-IL15/pSB CAR-T (25.4%), and the minicircle CD19RCD137/pSB CAR-T (35.12%) was higher than that of the iCasp9-IL15/pSB CAR-T (23.48%) with p<0.05. Cell proliferation results (4 days) with IL-2 (100U/mL) demonstrated that the PBMC population retained its function after the transposition. Conclusion: Transposition of CAR-T cell blocks based on the second- and fourth-generation plasmid and minicircle were successfully conducted that created a foundation for proliferation experiments and testing T cell function.

To evaluate knowledge changing of military doctors after training on medical response in disasters.

2025-04-01T00:50:34+00:00

Mục tiêu: Đánh giá thay đổi kiến thức của bác sỹ quân y về đáp ứng y tế trong thảm họa và so sánh, phân tích một số yếu tố liên quan sau tập huấn từ ngày 24 - 27/7/2024 tại Bệnh viện Bỏng Quốc gia Lê Hữu Trác, Học viện Quân y. Phương pháp nghiên cứu: So sánh, đánh giá kết quả trước và sau tập huấn bằng bộ câu hỏi trắc nghiệm được thiết kế sẵn trên 70 bác sỹ quân y các tuyến tham gia lớp tập huấn về đáp ứng y tế trong thảm họa. Kết quả:Phần lớn các bác sỹ chưa được tập huấn về y học thảm họa (88,57%). Kiến thức tổng hợp của các học viên tăng đáng kể sau tập huấn (70,71 ± 8,71% so với 86,28 ± 8,56%; p < 0,01). Tuy nhiên, kiến thức về đội cấp cứu và phân loại nạn nhân thảm họa vẫn ở mức thấp (21,43% và 24,29%). Thay đổi kiến thức được ghi nhận nhiều hơn ở nhóm bác sỹ trẻ < 40 tuổi (88,33% so với 82,36%; p < 0,05). Kết luận: Kiến thức về đáp ứng y tế trong thảm hoạ của học viên tăng đáng kể sau tập huấn; tỷ lệ trả lời đúng kiến thức về đáp ứng y tế trong thảm họa đều cải thiện ở nhiều nhóm nội dung. Không có sự khác biệt về kiến thức của các nhóm học viên về vị trí công tác, trình độ học vấn và đã được tập huấn hay chưa tập huấn. Tuy nhiên, thay đổi kiến thức được ghi nhận nhiều hơn đáng kể ở nhóm bác sỹ trẻ.

PREIMPLANTATION GENETIC DIAGNOSIS GLYCOGEN STORAGE DISEASE TYPE VI

2025-03-03T09:23:25+00:00

Glycogen storage disease type VI (GSD6; OMIM#232700), also known as Hers disease, is an autosomal recessive genetic disorder with an incidence of approximately 1 in 1,000,000 people worldwide. The disease is characterized by impaired glycogen degradation function in the liver, resulting from abnormalities in the PYGL gene that encodes for the enzyme phosphorylase - an essential enzyme in glycogenolysis. Individuals with the disease typically present early with symptoms such as hepatomegaly, developmental delay, and hypoglycemia… Disease prevention has become feasible due to pre-implantation genetic diagnosis (PGD), allowing couples carrying variant to have healthy children. Objectives: Report on Pre-Implantation Genetic Diagnosis of Glycogen Storage Disease Type VI (GSD6). Materials and methods: Based on the results of NGS sequencing of the affected son, Sanger sequencing was performed to identify the disease-causing variant in the blood samples of the father, mother, and child, along with 07 blastocyst biopsy samples from the couple. Additionally, linkage analysis using STR was conducted to provide the diagnostic results. Results: Pre-implantation genetic diagnosis for Glycogen Storage Disease type VI (GSD6) was successfully performed on a couple who had previously given birth to an affected child. Results: Two embryos were normal and did not carry the disease-causing variant; one embryo carried the variant PYGL: c.1334T>C (p.Leu445Pro), one embryo carried a deletion variant spanning exon 14 to exon 17 of the PYGL gene, and three affected embryos.

GIÁ TRỊ NGƯỠNG CẮT DIỆN TÍCH MỠ NỘI TẠNG TRÊN CHỤP CẮT LỚP VI TÍNH TRONG CHẨN ĐOÁN HỘI CHỨNG CHUYỂN HÓA Ở NGƯỜI KIỂM TRA SỨC KHỎE TẠI BỆNH VIỆN CHỢ RẪY

2025-03-10T00:30:09+00:00

Mục tiêu: Xác định giá trị ngưỡng cắt diện tích mỡ nội tạng (DTMNT) trên chụp cắt lớp vi tính (CLVT) trong chẩn đoán hội chứng chuyển hóa (HCCH). Phương pháp nghiên cứu:Nghiên cứu hồi cứu, mô tả cắt ngang trên 390 người đến khám từ tháng 3 - 4/2019 tại Trung tâm HECI, Bệnh viện Chợ Rẫy có tuổi trung bình 48,92 ± 10,59, nam giới chiếm đa số (n = 234/390). DTMNT đo trên chụp CLVT chỗ đo vòng bụng (VB) ngang mức rốn. HCCH được xác định theo sự thống nhất của Liên đoàn Đái tháo đường Quốc tế (IDF), Hiệp hội Tim mạch Hoa Kỳ (AHA) năm 2009. Giá trị đường cong ROC dùng xác định ngưỡng cắt của DTMNT. Kết quả:Ngưỡng cắt tối ưu DTMNT trong chẩn đoán HCCH là 85cm2 (độ nhạy 69,6%, độ đặc hiệu 72,9%) với giá trị diện tích dưới đường cong ROC (AUC) là 0,757; cao hơn so với VB và chỉ số khối cơ thể (body mass index - BMI) (0,739 và 0,639). Ngưỡng cắt ở nam giới là 107cm2 (độ nhạy 86,4%, độ đặc hiệu 68,3%); nữ giới là 61,8cm2 (độ nhạy 67,1%, độ đặc hiệu 68,8%). Ở nữ giới, ngưỡng cắt cũng tăng dần từ 29,2cm2 ở nhóm 18 - 39 tuổi, tăng lên 79,5cm2 ở nhóm 60 - 79 tuổi. Kết luận: DTMNT xác định trên chụp CLVT có giá trị chẩn đoán HCCH tốt hơn VB và BMI, với giá trị ngưỡng cắt tối ưu khác nhau theo giới tính và tuổi.

DIAGNOSTIC VALUE OF SHOX2 METHYLATION IN PLASMA FOR NON-SMALL CELL LUNG CANCER

2025-03-03T05:11:16+00:00

Objective: This study evaluates the methylation status of SHOX2 (mSHOX2) in peripheral blood and its diagnostic value for non-small cell lung cancer (NSCLC). Methods: Blood samples from 149 NSCLC patients and 100 healthy individuals were analyzed using semi-nested quantitative methylation-specific PCR (sqMSP). Diagnostic performance was assessed using ROC curve analysis and multivariate logistic regression. Results: mSHOX2 ΔCt was significantly lower in NSCLC patients than in healthy controls (p < 0.001), indicating higher methylation levels. ROC analysis showed AUC = 0.715, sensitivity 56.38%, and specificity 82.00%. Multivariate logistic regression confirmed that mSHOX2, age, and BMI were independent factors associated with NSCLC risk, while gender and smoking history were no longer significant after adjustment. Conclusion: mSHOX2 is a promising non-invasive biomarker for NSCLC detection. Further studies are needed to enhance diagnostic accuracy by integrating additional biomarkers.

RESEARCH ON THE VALUE OF METHYLATION OF THE SEPT9 GEN IN SERUM FOR THE DIAGNOSIS OF COLORECTAL CANCER

2025-03-25T06:58:08+00:00

Objectives: To study the value of methylation of the SEPT9 gene (mSEPT9) in peripheral blood for the diagnosis of colorectal cancer (CRC). Methods: A cross-sectional descriptive study involving 244 participants, including 92 healthy individuals undergoing health checks at Military Hospital 103 and 152 patients with CRC monitored at Military Hospital 103 and K Hospital from January 2021 to December 2024. The detection of mSEPT9 in peripheral blood was determined using the Nested-PCR technique with an extended sequence probe (ExBP). Results: The early detection value of CRC using mSEPT9 differentiates CRC patients from healthy individuals with a sensitivity of 76.31%, specificity of 89,13%, positive predictive value of 92,06%, and negative predictive value of 69,49%. ROC curve analysis recorded an AUC of 0,827 for the diagnostic value of CRC using mSEPT9, with p < 0.0001. Individuals with positive mSEPT9 are at a 26,42 times higher risk of CRC compared to those with negative mSEPT9 (p < 0.0001). In the CRC group, patients with positive mSEPT9 have a 3,719 times higher risk of progressing to late-stage disease compared to those with negative mSEPT9 (p < 0.001). Conclusion: mSEPT9 demonstrates high sensitivity and specificity as a diagnostic tool for CRC and its convenience and non-invasive nature play a role in improving the quality of early screening for CRC. Keywords: Colorectal cancer, SEPT9 gene methylation.

ĐÁNH GIÁ KẾT QUẢ SÀNG LỌC TRƯỚC SINH KHÔNG XÂM LẤN PHÁT HIỆN SỚM LỆCH BỘI NHIỄM SẮC THỂ THAI TRÊN CÁC THAI PHỤ CÓ NGUY CƠ CAO BẰNG PHƯƠNG PHÁP LÀM GIÀU DNA THAI TỰ DO TRONG MÁU MẸ

2025-04-08T15:16:52+00:00

EVALUATION OF THE RESULTS OF NON-INVASIVE PRENATAL SCREENING IN EARLY TO DETECT FETAL ANEUPLOIDIES IN HIGH-RISK PREGNANT WOMEN BY METHOD OF ENRICHMENT FREE FETAL DNA IN THE MOTHER'S BLOOD Dao The Anh1,3, Dang Tien Trương, Ho Sy Hung1,2, Nguyen Duy Bac3 1 Hanoi Medical University, 2 National Hospital of Obtetrics and Gynecology, Vietnamese Military University. Summary Introduction: Today, prenatal screening and diagnosis will help reduce the rate of babies with birth defects and perinatal death. Traditional screening tests have low sensitivity and specificity and a high false-positive rate. Recently, the non-invasive prenatal screening test (NIPT) that analyzes cffDNA (free fetal DNA) in pregnant women's blood has been introduced to help early detect syndromes related to abnormalities in the number of chromosomes in the fetus with high accuracy, simple sample collection process, safe for pregnant women and fetuses. This study aims to evaluate the results of the improved NIPT method that enriches free fetal DNA in maternal blood in screening for some common fetal chromosomal aneuploidies. Methods: Comparative cross-sectional descriptive study. The study subjects included 200 pregnant women with a high risk of having an aneuploid fetus examined at Hanoi Obstetrics and Gynecology Hospital to collect data, take serum samples to extract cffDNA and conduct analysis using the explication method. In order to detect abnormalities in the new generation, pregnant women undergo amniocentesis for chromosomal analysis after 16 weeks of pregnancy. NIPT. Results will be compared with chromosome results. Result: Average maternal age was 29.9 ± 5.7 years, average gestational age was 15.6 ± 3.3 weeks. The rate of patients with common chromosomal disorders T13, T18, T21, Monosomy X, Trisomy sex chromosomes reached 0,2% respectively; 1,9%; 4,8%; 1,9%; 2,2%. The sensitivity of NIPT in detecting trisomy 13, 18, 21 is 93,3%, sex chromosome is 88,99%, specificity is 99%, sex chromosome is 99%. Conclusion: NIPT is optimized by enriching free fetal DNA in maternal blood is a non-invasive prenatal screening test with high sensitivity and specificity in screening for some common chromosomal aneuploidies such as trisomy 13, 18, 21, and sex chromosomes.

EVALUATION OF SOME PROGNOSTIC PATHOLOGICAL FEATURES IN TUMOR MICROENVIRONMENT IN COLORECTAL ADENOCARCINOMA

2025-02-14T14:35:07+00:00

Objective: To evaluate the relationship between the tumor-infiltrating lymphocytes (TIL) and the tumor budding with prognostic pathological features in colorectal adenocarcinoma patients. Subjects and methods: A retrospective cross-sectional study of 176 colorectal adenocarcinoma patients at Military Hospital 103 from April 2021 to June 2024. TIL was evaluated according to ITWG 2014 and tumor budding according to ITBCC 2016 criteria. Results:The mean age was 66.03 ± 13.43; male/female ratio was 1.67/1. Low-grade TIL predominated (70.46%), while high-grade tumor budding accounted for 10.2%. Low-grade TIL was significantly associated with advanced tumor stage (p=0.015). High-grade tumor budding was correlated with poor differentiation (p=0.038), vascular (p<0.001) and perineural invasion (p=0.012). Conclusion: Tumor- infiltrating lymphocytes and tumor budding are closely associated with poor prognostic pathological features, confirming their roles as novel prognostic factors in colorectal adenocarcinoma.

CHẨN ĐOÁN GIẢI PHẪU BỆNH SINH THIẾT THẬN GHÉP THEO HỆ THỐNG PHÂN LOẠI BANFF NĂM 2022

2025-03-06T03:25:25+00:00

Objectives: The study aims to classify renal allograft pathology based on the updated 2022 Banff Classification. Methods: A cross-sectional descriptive study involving 195 patients who underwent renal allograft biopsy at Military Hospital 103 from January 2021 to December 2024. Results: Male patients accounted for 77.43%, with an average age of 38,94 ± 10,66 years. Living donors contributed to 98.98% of the cases. The most common cause of renal allograft dysfunction was rejection (49.23%), including antibody-mediated rejection (AMR) and T-cell-mediated rejection (TCMR). The earliest histological lesions were microvascular inflammation/injury (MVI) and acute AMR, occurring at an average of 0.13±0,07 and 0.58±1.19 months, respectively, while focal segmental glomerulosclerosis (FSGS) appeared the latest, at an average of 92±86.1 months. Patients of category 1 had mild increases in serum creatinine, while patients of category 6 had the highest creatinine levels. Conclusions: The timing of the development of various etiologies and the use of prebiopsy serum creatinine levels can help in the prediction of etiology of renal dysfunction. However, due to the considerable diversity of allograft pathologies and ongoing updates in new classification, applying the 2022 Banff one is essential.

ASSESSMENT OF PHARYNGEAL AIRWAY DIMENSIONS IN CONE-BEAM COMPUTED TOMOGRAPHY IN TEMPOROMANDIBULAR DISORDERS PATIENTS AT NATIONAL HOSPITAL OF ODONTO-STOMATOLOGY HANOI IN 2024

2025-03-30T14:47:10+00:00

Mục tiêu: Khảo sát kích thước đường thở vùng hầu trên người bệnh (NB) rối loạn thái dương hàm (temporomandibular disorders - TMDs). Phương pháp nghiên cứu: Nghiên cứu tiến cứu, mô tả cắt ngang trên phim chụp chùm tia hình nón (cone-beam computed tomography - CBCT) của 30 NB TMDs tại Bệnh viện Răng Hàm Mặt Trung ương Hà Nội. Kết quả: Diện tích mặt cắt ngang nhỏ nhất (CASmin): Or - CASmin (269,41 ± 107,94mm2) với khoảng cách bên (LR = 25,93 ± 6,49); khoảng cách trước sau (AP = 12,74 ± 3,44mm). Thể tích đường thở vùng hầu là 28,874 ± 8,326cm3. Chiều dài đường thở vùng hầu là 69,41 ± 6,73mm. Kết luận: Trên NB TMDs diện tích mặt cắt ngang nhỏ nhất nằm ở vùng hầu miệng, trong đó NB nam có kích thước lớn hơn NB nữ; giá trị số khối cơ thể (body mass index - BMI) cao có thể gây giảm kích thước đường thở.

SURVEY ON THE COMPLICATION RATE OF ENOXAPARIN INJECTION AND RELATED FACTORS IN PATIENTS AT THE INTENSIVE CARE UNIT

2025-03-02T13:10:09+00:00

Objective: The study was conducted to describe the rate of complications in patients using subcutaneous Enoxaparin and investigate factors associated with the risk of complications in the Emergency and Intensive Care Unit of Hanoi Medical University Hospital in 2024. Methods: A prospective observational study was performed on 55 intensive care patients receiving Enoxaparin. Results: The proportion of patients experiencing adverse effects from the drug was 90.9%. Of these, seven cases (14%) presented both systemic and local complications, while 43 cases (86%) had local complications. Conclusion: Factors associated with systemic complications included a history of bleeding, changes in glomerular filtration rate during treatment, a history of heart failure, and twice-daily dosing (p < 0.05). Factors related to complications at the injection site were age, weight, length of hospital stay, duration of drug use, history of hypertension, diabetes mellitus, and twice-daily dosing (p < 0.05).

ASSOCIATION BETWEEN DISEASE TYPE AND CLINICAL, PARACLINICAL CHARACTERISTICS, AND DISEASE STAGE IN PATIENTS WITH MULTIPLE MYELOMA

2025-03-06T08:20:46+00:00

Objective: This study aims to examine the relationship between disease type and clinical, paraclinical characteristics, as well as disease stage, in patients diagnosed with multiple myeloma (MM) at Military Hospital 103 from January 2016 to December 2024. Subjects and Methods: A cross-sectional descriptive study was conducted on 98 patients diagnosed with MM at Military Hospital 103. Results: The majority of patients had the IgG disease type (53.1%), followed by IgA (28.6%), Light Chain (12.2%), and other types (6.1%). Statistically significant differences were observed between disease types in median values of age, white blood cell count, platelet count, bone marrow plasmocyte ratio, and hemoglobin level group (p < 0.05). Similarly, variations in albumin concentration, Beta-2 Microglobulin levels, and ISS disease stage were also found among different disease types, with p-values of 0.016, 0.039, and 0.04, respectively. Among them, IgA had the highest proportion of patients with albumin levels below 35 g/L (85.7%), whereas Light Chain had the highest proportion with albumin ≥ 35 g/L (66.7%). Regarding ISS disease stage: IgG was most commonly observed in stage ISS II (57.7%), while IgA had the highest proportion in stage ISS III (50%). Conclusion: Disease type is associated with age, peripheral blood cell indices and ISS disease stage in patients with MM.

CLINICAL AND PARACLINICAL CHARACTERISTICS OF PATIENTS UNDERGOING OVARIAN STIMULATION USING THE ANTAGONIST PROTOCOL WITH DELTA-FOLLITROPIN (REKOVELLE)

2025-03-25T07:37:03+00:00

Objective:To evaluate the clinical and paraclinical characteristics of patients undergoing ovarian stimulation using the Antagonist protocol with Delta-follitropin (Rekovelle). Subjects and Methods:The study was conducted on 71 infertile couples treated at the Military Institute of Clinical Embryology from August 2023 to December 2024. The inclusion criteria were patients undergoing in vitro fertilization (IVF) with ovarian stimulation using Rekovelle, who agreed to participate in the study. Exclusion criteria included patients with endocrine disorders such as pituitary adenoma, hypothalamic tumors, thyroid dysfunction, breast tumors, ovarian tumors, uterine fibroids, genital malformations, as well as cases involving egg donation or egg preservation. The study employed a combination of retrospective and prospective methods with a cross-sectional descriptive design. Results:The average age of the patients was 36.31 ± 4.58 years. The rate of secondary infertility was 78.9%, while primary infertility accounted for 21.1%. The duration of infertility ranged from 1 to 14 years, with 70.4% of patients experiencing infertility for less than 5 years. The number of IVF cycles ranged from 1 to 4, with 1–2 cycles accounting for 81.7%. The average BMI of the patients was 21.78, and the AMH level was 1.33 (0.81–2.34) ng/mL. AMH levels varied by age group. FSH levels ranged from 3.01 to 17.1, while LH levels ranged from 2.46 to 11.48. The proportion of patients with an antral follicle count (AFC) of more than 5 follicles was 84.5%, while those with fewer than 5 follicles accounted for 15.5%. On day 2 of the menstrual cycle, the endometrial thickness was less than 8 mm in the majority of patients (80.3%), 8–12 mm in 18.3%, and more than 12 mm in 1.4%. Conclusion: The study evaluated the clinical and paraclinical characteristics of patients using the Antagonist protocol with Delta-follitropin (Rekovelle). These findings provide a basis for further research and evaluation in studies involving infertile patients using this protocol.

THE RESULTS OF PLASMA EXCHANGE IN HYPERTRIGLYCERIDEMIA- INDUCED PANCREATITIS PATIENTS

2025-04-03T08:29:16+00:00

Objectives: This study was conducted to evaluate the effectiveness of plasma exchange in hypertriglyceridemia-inducted pancreatitis patients. Methods: There are twenty- five patients who met the selection criteria of the study. Results: The male has 80,0%, the average age was 42,4 ± 9,2. Alcohol abuse, prior history of acute pancreatitis, and dyslipidemia were the most common medical conditions in history (52,0; 52,0, and 40,0%). The ratio of severity pancreatitis patients (Balthazar E) was 64,0%, and the average CTSI score was 4,74 (SD: 2,03). The length of intensive care unit stay was 4,9 days and hospital stay was 9,9 days. The average total hospital cost was 48 million VND/patient. The majority of patients have one time of TPE with 88% and 56% of them have minor allergies. Before and post-TPE, triglyceride levels decreased by an average of 45,70 mmol/L (82,5%) and the average triglyceride level was 55,92 mmol/L (93,5%) from hospitalization to discharge.Conclusion: Plasma exchange is an effective and safe treatment for patients with acute pancreatitis caused by hypertriglyceridemia.

ANTI – HLA ANTIBODIES BEFORE TRANSPLANTATION AND ASSOCIATION WITH SOME FACTORS IN PATIENT BEFORE KIDNEY TRANSPLANTATION

2025-04-08T09:02:54+00:00

Objectives: Survey of anti-HLA antibodies (PRA - Panel Reactive Antibodies) before transplanation and study of the relationship with some factors in patients before kidney transplant. Methods: Cross-sectional descriptive study in 152 patients receiving a kidney from a living donor, and post-transplant follow-up at Military Hospital 103, Vietnam Military Medical University (from April 2018 to April 2021). Results: The proportion of patients with positive anti-HLA antibodies before transplantation was 31.6%. Female patients had an average % of anti-HLA antibodies, and the rate of anti-HLA antibodies ≥ 10% was higher than male patients (p < 0.05). Blood transfusion and pregnancy are associated with developing anti-HLA antibodies. The risk of developing anti-HLA antibodies in the blood-transfusion group was higher than in the non-transfusion group (OR=2.793; p < 0.01). There was no correlation between pre-transplant HLA antibodies and age (p > 0.05). Conclusion: Pre-transplant PRA appears at a high rate (31.6%). Blood transfusion and pregnancy are risk factors for developing anti-HLA antibodies before kidney transplantation.

CLINICAL, PARACLINICAL CHARACTERISTICS AND INFLAMMATION-SCORES IN LATE STAGE NON-SMALL CELL LUNG CANCER

2025-04-08T01:12:08+00:00

Abstract Objectives: To describe clinical and paraclinical characteristics and some inflammatory-scores (NLR, PLS, SII) in late stage non-small cell lung cancer patients at Military Hospital 103. Methods: A retrospective and prospective, crosssectional study on 109 inpatients diagnosed with late stage non-small cell lung cancer at the Respiratory Center, Military Hospital 103 from January 2019 to September 2024. Results: The mean age of patiens were 65.43 ± 12.09 years, with a higher proportion of males. Patients have a variety of clinical symptoms, most common subjective symptoms were cough (86.33%) and chest pain (53.33%). The most common sites of metastasis were the pleura (42.20%), contralateral lung (22.93%), abdomen (19.27%) and bones (18.35%). 79,82% of patients were in stage IV, and adenocarcinoma accounted for 75.23% of the cases. On chest CT, the most frequent findings were pleural effusion (50/109) and round-shaped opacity (92/109). The median value of NLR was 3.467, with a range of 0.739 to 36.943. The median values for PLR and SII were 166.379 and 981.167, respectively, with PLR ranging from 35.494 to 1127.027 and SII from 232.214 to 14831.676. Conclusion: Patients with late stage non-small cell lung cancer were higher rate in male, high mean age with diversified clinical symptoms, predominantly adenocarcinoma, and typically presents with round-shaped opacity on chest CT. The median values of NLR, PLR, and SII were 3.467, 166.379, and 981.167, respectively.

Early evaluation of results using DynamX bioadaptor coated with Novolimus in the treatment of coronary artery disease at E Hospital

2025-03-12T07:20:28+00:00

Objective: To evaluate the early outcomes of using DynamX bioadaptor coated with novolimus in the treatment of coronary artery disease. Methods: Prospective, descriptive study without a control group. Results: 70 patients were enrolled in the study. The mean age was 70.4±7,9 years. Males accounted for 61.4%. Most were hospitalized with a diagnosis of acute coronary syndrome (89%). The most common lesion was the left anterior descending artery (47.1%) with a calcification and tortuosity rate of 14.2% and 7.1%, respectively. The clinical success rate was 98.6%. No major complications were reported during the procedure. There was a significant improvement in chest pain, shortness of breath, and cardiac function at 1 and 3 months follow-up. No major cardiovascular events were reported at 1 and 3 months follow-up. Conclusion: The study demonstrated the safety of DynamX bioadaptor coated with novolimus in the early treatment of coronary artery disease.

NGHIÊN CỨU GIÁ TRỊ CỦA ĐỘ THANH THẢI LACTATE MÁU TRONG TIÊN LƯỢNG TỬ VONG Ở BỆNH NHÂN SỐC NHIỄM KHUẨN

2025-04-14T02:13:39+00:00

Objective: To investigate the value of lactate clearance in predicting mortality in patients with septic shock. Method: septic shock was diagnosed according to sepsis 3-2016, lactate was quantified at the time of admission, 12 hours and 24 hours later and monitored until stable discharge or death. Lactate clearance was calculated according to the formula: lactate clearance = (initial blood lactate - blood lactate at the time of survey) x 100% / initial blood lactate. Compare lactate clearance between the 2 groups of survival and death, draw ROC curve, calculate area under the curve in predicting mortality. Results: 92 patients met the criteria to participate in the study, the mortality rate in patients with septic shock was 52.2%. Mean blood lactate at admission, 12 hours and 24 hours were 4.8 ± 3.4 mmol/l; 3.9 ± 2.9 mmol/l and 3.0 ± 2.9 mmol/l, respectively. Lactate clearance at 12 hours and 24 hours was 10.1 ± 5.6% and 5.1 ± 11.4%, respectively. Lactate clearance in the survival group was statistically significantly higher than in the death group, p < 0.05. Lactate clearance at 12 and 24 hours after admission was valuable in predicting mortality, corresponding to an area under the curve of 0.64 (cut off 17.8, sensitivity 62.5%, specificity 69.4%) and 0.77 (cut off 15.6, sensitivity 79.2%, specificity 61.1%), p < 0.05. Conclusion: Lactate clearance at 12 and 24 hours after admission was valuable in predicting mortality in patients with septic shock.

ĐÁNH GIÁ TÁC DỤNG CỦA LASER CHÂM KẾT HỢP XOA BÓP BẤM HUYỆT ĐIỀU TRỊ VIÊM QUANH KHỚP VAI THỂ ĐƠN THUẦN

2025-05-07T09:02:59+00:00

Objectives:To evaluate the effect of laser acupuncture combined with acupressure massage on treating periarthritis of the shoulder.Method: A clinical, prospective study comparing before and after treatment, with a control group. Sixty patients with periarthritis of the shoulder were enrolled. After 15 days of treatment, the average VAS score decreased from 5.87 ± 1.00 to 1.83 ± 0.92 (p < 0.01). The average range of motion of the shoulder joint in all three movements: abduction, internal rotation, and external rotation increased. Conclusion: Laser acupuncture combined with acupressure massage effectively improves pain and range of motion in patients with shoulder periarthritis.

OUTPATIENT TREATMENT OUTCOMES OF ALLERGIC RHINITIS WITH INTRANASAL BUDESONIDE AT MILITARY HOSPITAL 103

2025-02-21T07:55:07+00:00

Objective: To evaluate the improvement in clinical symptoms and quality of life (QoL) in patients with allergic rhinitis (AR) undergoing outpatient treatment with intranasal Budesonide. Methods: A prospective, case-by-case descriptive study was conducted on 61 patients diagnosed with AR at Military Hospital 103 from October 2023 to December 2024. Results: House dust was the most common allergen, accounting for 83.6% of cases. After three months of treatment: 77.0% of patients had complete resolution of nasal itching; 73.8% had no further sneezing episodes; 78.7% experienced cessation of rhinorrhea; and 45.9% achieved full relief from nasal congestion. Additionally, 72.1% exhibited normalization of the nasal mucosa, and 83.6% showed restored inferior turbinate morphology. The mean QoL score improved significantly from 2.2 ± 0.6 to 0.8 ± 0.2, with a mean reduction of 1.4 ± 0.5 points, reflecting an overall improvement rate of 63.6%. Conclusion: Intranasal Budesonide showed marked improvement in both clinical symptoms and quality of life in patients with allergic rhinitis after three months of treatment.

PRIMARY SPLENIC ECTOPIC PREGNANCY: A CASE REPORT AT MILITARY HOSPITAL 175

2025-03-07T01:11:14+00:00

Primary splenic ectopic pregnancy (SEP) is an extremely rare condition that is difficult to diagnose and one of the leading causes of maternal mortality during the first trimester of pregnancy. Early diagnosis allows for the selection of various conservative treatment methods instead of splenectomy, as was common in the past. In this report, we present a case of primary splenic ectopic pregnancy (SEP) diagnosed and treated at Military Hospital 175. The patient was admitted to the hospital due to abdominal pain. Transvaginal ultrasound revealed intra-abdominal fluid with echoes, a normal uterus, and no gestational sac in the uterine cavity. Laboratory tests showed significantly elevated βHCG levels. The patient subsequently underwent contrast-enhanced computed tomography (CT), which revealed a rim-enhancing mass at the upper pole of the spleen accompanied by perisplenic hematoma. The patient was treated with an open surgical splenectomy. Imaging techniques must be promptly performed in cases of abdominal pain and missed periods in women of reproductive age to facilitate early diagnosis and appropriate treatment.

NGHIÊN CỨU MỐI LIÊN QUAN CỦA NỒNG ĐỘ MMP-9 VÀ TIMP-1 HUYẾT TƯƠNG Ở THAI PHỤ TIỀN SẢN GIẬT

2025-03-27T08:37:23+00:00

Objective: Determine the concentrations of MMP-9 and TIMP-1 in the plasma of normal pregnant women and pre-eclamptic pregnant women, and find out the relationship between MMP-9 and TIMP-1 concentrations in plasma with some symptoms of pre-eclampsia. Subjects and methods: including two groups of pregnant women: normal pregnant women (control group n -120) and preeclamptic pregnant women (disease group n = 120). MMP-9 and TIMP-1 concentrations were determined by ELISA technique.Results: MMP-9 concentration in the plasma of preeclamptic pregnant women was 403.0 (207.6 – 794.1) ng/mL, lower than that of normal pregnant women, 608.6 (238.9 – 1212.0) ng/mL (p = 0.032). The concentration of TIMP-1 in the plasma of preeclamptic pregnant women is 196.8 (125.0 – 272.9) ng/mL and lower than in normal pregnant women is 225.8 (141.5 – 314.0) ng/mL (p = 0.14). The MMP-9/TIMP-1 ratio in preeclamptic 2.21 (1.39 – 3.20) was lower than normal pregnant women 2.83 (1.71 – 4.59), respectively, p = 0.008. There was a difference in plasma MMP-9 and TIMP-1 concentrations between the group of pregnant women with preeclampsia with grade 1 hypertension and pregnant women with grade 2 hypertension (p < 0.05). Conclusion: plasma MMP-9 concentration and MMP-9/TIMP-1 ratio in normal pregnant women are lower than in pre-eclamptic pregnant women. There is a relationship between plasma MMP-9 and TIMP-1 concentrations and the degree of hypertension in preeclamptic pregnant women.

EVALUATION OF THE RESULTS OF NEURONAVIGATION- GUIDED ASPIRATION SURGERY FOR CEREBRAL HEMISPHERE ABSCESS AT MILITARY HOSPITAL 103

2025-02-18T08:58:50+00:00

Objective: To evaluate the outcomes of neuronavigation- guided aspiration surgery in patients with cerebral hemisphere abscess (CHA) at Military Hospital 103. Methods: A descriptive study combining retrospective and prospective analysis was conducted on 34 patients diagnosed with CHA who underwent navigation- guided aspiration surgery from January 2019 to June 2024. Patients were followed up for 3 months postoperatively. Results: The mean abscess cavity diameter and midline shift at discharge were 2.09 ± 2.91 mm and 2.12 ± 0.73 mm, respectively, which were significantly smaller than preoperative values (p = 0.001 and p = 0.01). After 3 months, 55.9% of patients achieved good recovery (GOS grade IV, V). There was a correlation between postoperative recovery (measured by GOS) and preoperative Glasgow Coma Scale (GCS) scores as well as the presence of intraventricular rupture of the abscess. Streptococcus was the most isolated bacterial strain, followed by Staphylococcus and Klebsiella. Conclusion: The abscess cavity diameter and midline shift at discharge were significantly smaller than preoperative values. Preoperative GCS scores and the presence of intraventricular abscess rupture were associated with neurological recovery at 3 months. Streptococcus was the most frequently isolated pathogen.

NHẬN XÉT GIÁ TRỊ TIÊN LƯỢNG CỦA MỘT SỐ ĐẶC ĐIỂM LÂM SÀNG VÀ TỔN THƯƠNG NÃO LÚC NHẬP VIỆN Ở BỆNH NHÂN CHẤN THƯƠNG SỌ NÃO NẶNG TẠI BỆNH VIỆN QUÂN Y 105

2025-03-25T08:06:43+00:00

Objective: Objective: To study the prognostic value of some clinical symptoms at admission and brain damage on the treatment outcome of patients with severe traumatic brain injury (TBI). Methods: The study was prospectively conducted on 68 patients with severe traumatic brain injury treated at Military Hospital 105 from April 2024 to March 2025. Clinical and paraclinical signs were collected according to the study medical records, and the progression to treatment outcome was monitored. Treatment outcome was expressed by the overall mortality rate and mortality between the groups: mechanical ventilation and non-mechanical ventilation; deep coma and shallow coma; dilated and non-dilated pupils; epidural hematoma and non-epidural hematoma; brainstem hemorrhage and non-brainstem hemorrhage; surgery and non-surgery. Results: The overall mortality rate was 25%. The mortality rate did not differ between men and women, the group requiring mechanical ventilation upon admission and the group not requiring mechanical ventilation upon admission. But the mortality rate increased in the deep coma group (p=0.023; OR=12.3; CI: 3.84 - 14.63); the dilated pupil group (p=0.025; OR=18; CI: 11.21 - 19.143); and the brainstem hemorrhage group (p=0.001; OR=6.67; (CI: 1.14 - 8.23). In contrast, the mortality rate was lower in the simple epidural hematoma group (OR = 0.012) and the group of patients with indications and undergoing craniotomy, the non-surgical group was higher than the surgical group (p < 0.05). Conclusion: The mortality rate increased in patients with severe traumatic brain injury with deep coma, dilated pupil, basal cistern effacement and brainstem hemorrhage.

INITIAL OUTCOMES OF PROSTATIC ARTERY EMBOLIZATION VIA RADIAL ARTERY ACCESS FOR BENIGN PROSTATIC HYPERPLASIA

2025-04-16T01:03:16+00:00

Objectives: To describe the initial outcomes of prostatic artery embolization (PAE) via the radial artery at Hanoi Medical University Hospital. Methods: a prospective descriptive study Results: A total of 46 patients underwent prostatic artery embolization via the radial artery, with a mean age of 71.8 ± 9.0 years. The most common symptom was dysuria (63.0%), followed by nocturia (43.5%). Technical success was achieved in 100% of cases. The mean procedure time was 89.4 minutes, with an average fluoroscopy time of 38.1 minutes. No intra-procedural complications were observed in any patient. Pain during glue injection was commonly reported, including penile pain (41.3%) and scrotal pain (4.3%). One patient (2.2%) experienced rectal bleeding due to embolic material migration into the rectum. Rectal endoscopy revealed focal mucosal congestion, but symptoms resolved spontaneously within one week without the need for medical or surgical intervention. Conclusion: Prostatic artery embolization via the radial artery is a highly effective technique for the treatment of benign prostatic hyperplasia.